![Immunohistochemistry: Ferritin Antibody (12H1) [NBP3-26568] - Image of Ferritin Antibody (12H1) diluted at 1:50 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26568_mouse-ferritin-mab-12h1-26220241447157.jpg "Immunohistochemistry: Ferritin Antibody (12H1) [NBP3-26568] - Image of Ferritin Antibody (12H1) diluted at 1:50 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, Flow, IHC |
| Clone | 12H1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Additional Information | Recombinant monoclonal antibody expressed in HEK293F cells |
| Immunogen | Recombinant Human Ferritin protein [UniProt P02794] |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FTH1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20 to -70C. Avoid freeze-thaw cycles. |
| Buffer | 50% Glycerol, 0.01M PBS, PH 7.4 |
| Preservative | 0.03% Proclin 300 |
| Purity | Affinity purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | FTH1 |
![Immunohistochemistry: Ferritin Antibody (12H1) [NBP3-26568] - Image of Ferritin Antibody (12H1) diluted at 1:50 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26568_mouse-ferritin-mab-12h1-27220248573313.jpg "Immunohistochemistry: Ferritin Antibody (12H1) [NBP3-26568] - Image of Ferritin Antibody (12H1) diluted at 1:50 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Ferritin Antibody (12H1) [NBP3-26568] - Positive Western Blot detected in: U251 whole cell lysate, JK whole cell lysate. All lanes: Ferritin Antibody at 1: 500 Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution. Predicted band size: 22 kDa Observed band size: 22 kDa](http://images.novusbio.com/fullsize/nbp3-26568_mouse-ferritin-mab-12h1-282202411485815.jpg "Western Blot: Ferritin Antibody (12H1) [NBP3-26568] - Positive Western Blot detected in: U251 whole cell lysate, JK whole cell lysate. All lanes: Ferritin Antibody at 1: 500 Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution. Predicted band size: 22 kDa Observed band size: 22 kDa")
![Flow Cytometry: Ferritin Antibody (12H1) [NBP3-26568] - Overlay Peak curve showing 293 cells stained with Ferritin Antibody (12H1) (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45 min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG (H+L) at 1/200 dilution for 35 min at 4C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26568_mouse-ferritin-mab-12h1-282202414312738.jpg "Flow Cytometry: Ferritin Antibody (12H1) [NBP3-26568] - Overlay Peak curve showing 293 cells stained with Ferritin Antibody (12H1) (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45 min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG (H+L) at 1/200 dilution for 35 min at 4C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.")
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