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FDC Antibody (CNA.42)

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Immunohistochemistry-Paraffin: FDC Antibody (CNA.42) [NBP1-43118] - Formalin-fixed paraffin embedded human tonsil using 1 ug/mL Mouse IgM Isotype Control (left) or 1 ug/mL Anti-Human FDC Purified (right) followed by anti-Mouse IgG Biotin and DAB visualization. Nuclei are counterstained with hematoxylin.
Immunohistochemistry-Paraffin: FDC Antibody (CNA.42) [NBP1-43118] - Formalin-fixed paraffin embedded human tonsil using 1 ug/mL Mouse IgM Isotype Control (left) or 1 ug/mL Anti-Human FDC Purified (right) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC
Clone
CNA.42
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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FDC Antibody (CNA.42) Summary

Immunogen
The immunogen for this antibody was follicular dendritic cells.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
CMD1B
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
This CNA.42 antibody has been tested by flow cytometric of human cell line and western blotting. This can be used at less than or equal to 0.5 ug per million cells in a 100 ul total staining volume or 2ug/ml (for western blotting).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for FDC Antibody (CNA.42)

  • cardiomyopathy, dilated 1B (autosomal dominant)
  • CMPD1
  • FDC

Background

The monoclonal antibody CNA.42 recognizes a unique carbohydrate epitope found on follicular dendritic cells (FDCs) from a variety of species. FDCs are large cells in germinal centers (GCs) of primary and lymphoid tissues. They present non-processed antigen and switch off apoptosis of B cells through a selection process resulting in selection of memory B cells. FDCs express many B cell markers such as CD21 (long isoform), CD23 and CD35. A role for FDCs as been shown in various infectious diseases such as AIDS and transmissible spongiform encephalitis. Staining with this CNA.42 monoclonal antibody is mainly restricted to the plasma membrane but in limited cases may also be cytoplasmic (as seen in mast cells). This monoclonal has been shown to be a useful reagent for both flow cytometry and IHC analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CMD1B