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Erythropoietin R Antibody (3D10)

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Western Blot: Erythropoietin R Antibody (3D10) [H00002057-M01] - Analysis of EPOR expression in transfected 293T cell line by EPOR monoclonal antibody (M01), clone 3D10.Lane 1: EPOR transfected lysate (Predicted MW: 55.1 KDa).Lane 2: Non-transfected lysate.
Western Blot: Erythropoietin R Antibody (3D10) [H00002057-M01] - Analysis of EPOR expression in transfected 293T cell line by EPOR monoclonal antibody (M01), clone 3D10.Lane 1: EPOR transfected lysate (Predicted MW: ...read more
Western Blot: Erythropoietin R Antibody (3D10) [H00002057-M01] - Western Blot analysis of EPOR expression in different cell lines.
Western Blot: Erythropoietin R Antibody (3D10) [H00002057-M01] - EPOR monoclonal antibody (M01), clone 3D10. Analysis of EPOR expression in HeLa.
Immunoprecipitation: Erythropoietin R Antibody (3D10) [H00002057-M01] - Analysis of EPOR transfected lysate using anti-EPOR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EPOR MaxPab rabbit ...read more
ELISA: Erythropoietin R Antibody (3D10) [H00002057-M01] - Detection limit for recombinant GST tagged EPOR is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IP
Clone
3D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Erythropoietin R Antibody (3D10) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
EPOR (NP_000112, 31 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PDPKFESKAALLAARGPEELLCFTERLEDLVCFWEEAASAGVGPGNYSFSYQLEDEPWKLCRLHQAPTARGAVRFWCSLPTADTSSFVPLELRVTAASGA
Specificity
EPOR - erythropoietin receptor
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EPOR
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Reviewed Applications
Read 1 Review rated 5
using
H00002057-M01 in the following applications:
Publications
Read Publication using H00002057-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Erythropoietin R Antibody (3D10)

  • EpoR
  • EPO-R
  • Erythropoietin R
  • erythropoietin receptor
  • MGC138358

Background

The erythropoietin receptor is a member of the cytokine receptor family. Upon erythropoietin binding, the erythropoietin receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Erythropoietin R
Antibody (3D10)
H00002057-M01
Erythropoietin R Antibody (3D10)
Species: Hu
Applications: WB, ELISA, IP

Publications for Erythropoietin R Antibody (H00002057-M01)(1)


Review for Erythropoietin R Antibody (H00002057-M01) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00002057-M01:
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(1)
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Images Ratings Applications Species Date Details
Western Blot Erythropoietin R H00002057-M01
Enlarge 		5
reviewed by:
zhang meng
 WB Human 11/17/2017
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Summary

ApplicationWestern Blot
Sample TestedHASMC human aortic smooth muscle cells
SpeciesHuman
Lot10145-3D10

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Recent Reviews

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Western Blot Erythropoietin R H00002057-M01
Enlarge
 zhang meng
11/17/2017
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol EPOR
Entrez
Uniprot