Orthogonal Strategies: Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in human thyroid gland and skeletal muscle tissues. Corresponding PDIA3 RNA-seq data are presented for ...read more
Genetic Strategies: Western Blot: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PDIA3 antibody. Remaining ...read more
Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in U251 cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are ...read more
Western Blot: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Lane 1: Marker [kDa]. Lane 2:Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in HeLa cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are ...read more
Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human cell line A431 showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes ...read more
Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in MCF7 cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are ...read more
Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in U2OS cell line with Anti-PDIA3 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. ...read more
Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human thyroid gland shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
This antibody was developed against Recombinant Protein corresponding to amino acids:PTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASIVGFFDDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEDK
Epitope
KKFISDKDASIVGFF
Predicted Species
Mouse (91%), Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PDIA3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified
Alternate Names for ERp57/PDIA3 Antibody (CL2444)
Disulfide isomerase ER-60
EC 5.3.4.1
endoplasmic reticulum P58
Endoplasmic reticulum resident protein 57
Endoplasmic reticulum resident protein 60
ER protein 60
ER60
ERp57
ERp5758 kDa glucose-regulated protein
ERp60
ERp6058 kDa microsomal protein
ERp61
glucose regulated protein, 58kDa
GRP57
GRP58
GRP58ER protein 57
HsT17083
P58
PDIA3
phospholipase C-alpha
PI-PLC
protein disulfide isomerase family A, member 3
protein disulfide isomerase-associated 3
protein disulfide-isomerase A3
Background
ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glucosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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