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Human ERK3/MAPK6 ELISA Kit (Colorimetric)

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ELISA: Human ERK3/MAPK6 ELISA Kit (Colorimetric) [NBP3-00379] - Samples were spiked with high concentrations of Human ERK3/MAPK6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human ERK3/MAPK6 ELISA Kit (Colorimetric) [NBP3-00379] - Samples were spiked with high concentrations of Human ERK3/MAPK6 and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Human ERK3/MAPK6 ELISA Kit (Colorimetric) [NBP3-00379] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

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Human ERK3/MAPK6 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human Human ERK3/MAPK6 in samples. No significant cross-reactivity or interference between Human Human ERK3/MAPK6 and analogues was observed.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
4.66%
Intra-Assay
4.79%
Spike Recovery
90 - 103%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
MAPK6

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human ERK3/MAPK6 ELISA Kit (Colorimetric)

  • DKFZp686F03189
  • EC 2.7.11
  • EC 2.7.11.24
  • ERK3
  • ERK-3
  • ERK3p97-MAPK
  • Extracellular signal-regulated kinase 3
  • extracellular signal-regulated kinase, p97
  • HsT17250
  • MAP kinase 6
  • MAP kinase isoform p97
  • MAPK 6
  • MAPK6
  • mitogen-activated protein kinase 6
  • p97MAPK
  • PRKM6
  • protein kinase, mitogen-activated 5
  • protein kinase, mitogen-activated 6

Background

ERK3 (Extracellular signal-regulated kinase 3, MAPK6, p97 MAPK) belongs to the Ser/Thr protein kinase family and is a member of the mitogen-activated protein (MAP) kinase superfamily. Unlike the other members of the MAPK family, ERK3 is a mitogen-activated protein kinase homologue that has SEG motif instead of conserved TXY motif in the activation loop (1). Also, unlike ERK1 and ERK2, it is ubiquitously expressed and localized in the nucleus of proliferating cells (2). Highly unstable, ERK3 is constitutively degraded during ubiquitin-proteasome pathway of proliferating cells (3). ERK3 has been linked to phosphorylation MAP2 and as a possible regulator during cell differentiation (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Human ERK3/MAPK6
ELISA Kit (Colorimetr ...
NBP3-00379
Human ERK3/MAPK6 ELISA Kit (Colorimetric)
Species: Hu
Applications: ELISA

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  Human ERK3/MAPK6 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol MAPK6