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Mouse ERK1 ELISA Kit (Colorimetric)

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ELISA: Mouse ERK1 ELISA Kit (Colorimetric) [NBP3-00430] - Samples were spiked with high concentrations of Mouse ERK1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Mouse ERK1 ELISA Kit (Colorimetric) [NBP3-00430] - Samples were spiked with high concentrations of Mouse ERK1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Mouse ERK1 ELISA Kit (Colorimetric) [NBP3-00430] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL

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Mouse ERK1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse ERK1 in samples. No significant cross-reactivity or interference between Mouse ERK1 and analogues was observed.
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
5.04%
Intra-Assay
5.6%
Spike Recovery
89 - 108%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
MAPK3

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse ERK1 ELISA Kit (Colorimetric)

  • EC 2.7.11
  • ERK1
  • ERK-1
  • ERK1p44-MAPK
  • ERT2
  • Extracellular signal-regulated kinase 1
  • extracellular signal-related kinase 1
  • HS44KDAP
  • HUMKER1A
  • Insulin-stimulated MAP2 kinase
  • MAP kinase 1
  • MAP kinase 3
  • MAP kinase isoform p44
  • MAPK 1
  • MAPK 3
  • MAPK3
  • MGC20180
  • Microtubule-associated protein 2 kinase
  • Mitogen-activated protein kinase 1
  • mitogen-activated protein kinase 3
  • P44ERK1
  • p44-ERK1
  • p44mapk
  • PRKM3
  • PRKM3EC 2.7.11.24

Background

p44 MAP Kinase (Erk1) is a member of a family of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth and differentiation (1,2). Erk1 regulates meiosis and mitosis by phosphorylating a number of transcription factors such as ELK-1. Activation of Erk1 occurs through phosphorylation of threonine 202 and tyrosine 204 (of human MAP kinase) (3). Erk1 is also known to autophosphorylate on tyrosine (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Contact Information

Product PDFs

  SDS
  Mouse ERK1 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol MAPK3