![Western Blot: ERGI3 Antibody [NBP2-16367] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ERGI3-Antibody-Western-Blot-NBP2-16367-img0001.jpg "Western Blot: ERGI3 Antibody [NBP2-16367] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE gel, diluted at 1:1000.")
| Reactivity | Hu, Mu, Rt, Bv, RbSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ERGI3. The exact sequence is proprietary. |
| Localization | Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein; Golgi apparatus; cis-Golgi network membrane; Multi-pass membrane protein; Endoplasmic reticulum membrane |
| Predicted Species | Mouse (96%), Rat (97%), Bovine (96%), Rabbit (97%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ERGIC3 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 43 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for ERGI3 Antibody (NBP2-16367)Find related products by research area.
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| Gene Symbol | ERGIC3 |
![Immunocytochemistry/Immunofluorescence: ERGI3 Antibody [NBP2-16367] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERGIC3 protein stained by ERGIC3 antibody diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ERGI3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16367-img0003.jpg "Immunocytochemistry/Immunofluorescence: ERGI3 Antibody [NBP2-16367] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERGIC3 protein stained by ERGIC3 antibody diluted at 1:1000. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: ERGI3 Antibody [NBP2-16367] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ERGI3-Antibody-Immunohistochemistry-Paraffin-NBP2-16367-img0002.jpg "Immunohistochemistry-Paraffin: ERGI3 Antibody [NBP2-16367] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")