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ER alpha/NR3A1 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human cerebral cortex, cervix, uterine, endometrium and gastrointestinal using Anti-ER alpha/NR3A1 ...read more
Western Blot: ER alpha/NR3A1 Antibody [NBP1-84826] - Analysis in control (vector only transfected HEK293T lysate) and ESR1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian ...read more
Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human uterine cervix shows strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human endometrium shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human small intestine shows no positivity in glandular cells as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ER alpha/NR3A1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYIT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ESR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Estrogen Receptor Protein (NBP1-84826PEP)
Publications
Read Publication using NBP1-84826.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%). Reactivity reported in scientific literature (PMID: 24403002)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ER alpha/NR3A1 Antibody

  • ER alpha
  • ER
  • Era
  • ER-alpha
  • ESR1
  • ESRESRA
  • Estradiol receptor
  • estrogen receptor 1
  • estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
  • estrogen receptor alpha delta 4 +49 isoform
  • estrogen receptor alpha delta 4*5,6,7*/654 isoform
  • estrogen receptor alpha
  • estrogen receptor
  • NR3A1
  • NR3A1DKFZp686N23123
  • Nuclear receptor subfamily 3 group A member 1

Background

Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation on serines, 104, 106, and 118 function to regulate the transcriptional activation of ER alpha (2). The phosphorylation of the human estrogen receptor (ER) serine residue at position 118 is required for full activity of the ER activation function 1 (AF-1) (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ER alpha/NR3A1 Antibody (NBP1-84826)(1)


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Bioinformatics

Gene Symbol ESR1
Entrez
Uniprot