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EPX Antibody (EPX/3908R) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC
Clone
EPX/3908R
Clonality
Monoclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-08476AF350
Alexa Fluor 405
NBP3-08476AF405
Alexa Fluor 488
NBP3-08476AF488
Alexa Fluor 532
NBP3-08476AF532
Alexa Fluor 594
NBP3-08476AF594
Alexa Fluor 647
NBP3-08476AF647
Alexa Fluor 700
NBP3-08476AF700
Alexa Fluor 750
NBP3-08476AF750
Allophycocyanin
NBP3-08476APC
Biotin
NBP3-08476B
DyLight 350
NBP3-08476UV
DyLight 405
NBP3-08476V
DyLight 488
NBP3-08476G
DyLight 550
NBP3-08476R
DyLight 594
NBP3-08476DL594
DyLight 650
NBP3-08476C
DyLight 680
NBP3-08476FR
DyLight 755
NBP3-08476IR
FITC
NBP3-08476F
HRP
NBP3-08476H
Janelia Fluor 525
NBP3-08476JF525
Janelia Fluor 549
NBP3-08476JF549
Janelia Fluor 646
NBP3-08476JF646
Janelia Fluor 669
NBP3-08476JF669
PE
NBP3-08476PE
PerCP
NBP3-08476PCP
Unconjugated
NBP3-07389-100ug
mFluor Violet 450 SE
NBP3-08476MFV450
mFluor Violet 500 SE
NBP3-08476MFV500
mFluor Violet 610 SE
NBP3-08476MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-07389-100ug
Azide and BSA Free
NBP3-08476
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EPX Antibody (EPX/3908R) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Human eosinophils from a patient with hypereosinophilic syndrome (Uniprot: P11678 )
Localization
Cytoplasmic, granular
Specificity
Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. AHE-1 recognizes human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. This monoclonal antibody stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO).
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
EPX
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for EPX Antibody (EPX/3908R) [CoraFluor™ 1]

  • EC 1.11.1.7
  • eosinophil peroxidase
  • EPOEPER
  • EPPEC 1.11.1
  • EPX-PEN

Background

The EPX gene is a member of the peroxidase gene family and is expressed in eosinophils. The encoded precursor protein is processed into covalently attached heavy and light chains to form the mature enzyme, which functions as an oxidant. The enzyme is release

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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EPX Antibody
(EPX/3908R) [CoraFluo ...
NBP3-08476CL1
EPX Antibody (EPX/3908R) [CoraFluor™ 1]
Species: Hu
Applications: Flow, ICC/IF, IHC

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol EPX