![Western Blot: EPHX1 Antibody [NBP1-33011] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/EPHX1-Antibody-Western-Blot-NBP1-33011-img0005.jpg "Western Blot: EPHX1 Antibody [NBP1-33011] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EPHX1. The exact sequence is proprietary. |
| Localization | Microsome membrane, Single-pass membrane protein, Endoplasmic reticulum membrane, Single-pass membrane protein |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EPHX1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 53 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
| Publication using NBP1-33011 | Applications | Species |
|---|---|---|
| Gautheron J, Morisseau C, Chung WK Et al. EPHX1 mutations cause a lipoatrophic diabetes syndrome due to impaired epoxide hydrolysis and increased cellular senescence eLife 2021-08-03 [PMID: 34342583] (WB, ICC/IF, Human) | WB, ICC/IF | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for EPHX1 Antibody (NBP1-33011)Find related products by research area.
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| Gene Symbol | EPHX1 |
![Immunocytochemistry/Immunofluorescence: EPHX1 Antibody [NBP1-33011] - Analysis of methanol-fixed HeLa, using EPHX1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/EPHX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33011-img0003.jpg "Immunocytochemistry/Immunofluorescence: EPHX1 Antibody [NBP1-33011] - Analysis of methanol-fixed HeLa, using EPHX1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.")
![Immunohistochemistry-Paraffin: EPHX1 Antibody [NBP1-33011] - Mouse liver. EPHX1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/EPHX1-Antibody-Immunohistochemistry-Paraffin-NBP1-33011-img0007.jpg "Immunohistochemistry-Paraffin: EPHX1 Antibody [NBP1-33011] - Mouse liver. EPHX1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: EPHX1 Antibody [NBP1-33011] - SG xenograft, using Epoxide hydrolase 1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/EPHX1-Antibody-Immunohistochemistry-Paraffin-NBP1-33011-img0004.jpg "Immunohistochemistry-Paraffin: EPHX1 Antibody [NBP1-33011] - SG xenograft, using Epoxide hydrolase 1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")