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EphA3 Antibody

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Western Blot: EphA3 Antibody [NBP2-16346] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] diluted at 1:1500. The HRP-conjugated anti-rabbit IgG ...read more
Immunohistochemistry-Paraffin: EphA3 Antibody [NBP2-16346] - Human gastric cancer, using Eph Receptor A3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Western Blot: EphA3 Antibody [NBP2-16346] - Mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] diluted at 1:1500. The HRP-conjugated anti-rabbit IgG ...read more
Western Blot: EphA3 Antibody [NBP2-16346] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] diluted at 1:1500. The HRP-conjugated anti-rabbit IgG ...read more
Western Blot: EphA3 Antibody [NBP2-16346] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] (NBP2-16346) diluted at 1:1500. The HRP-conjugated ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, ChSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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EphA3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EphA3. The exact sequence is proprietary.
Localization
Isoform 1: Cell membrane; Single-pass type I membrane protein; Isoform 2: Secreted
Predicted Species
Rat (96%), Porcine (97%), Bovine (97%), Chicken (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EPHA3
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for EphA3 Antibody

  • Cek4
  • EC 2.7.10
  • EC 2.7.10.1
  • EK4
  • EPH receptor A3
  • EphA3
  • EPH-like kinase 4
  • ephrin type-A receptor 3
  • ETK
  • Hek4
  • HEKETK1eph-like tyrosine kinase 1
  • human embryo kinase 1
  • TYRO4 protein tyrosine kinase
  • Tyro4
  • Tyrosine-protein kinase receptor ETK1
  • Tyrosine-protein kinase TYRO4

Background

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for EphA3 Antibody (NBP2-16346). (Showing 1 - of FAQ).

    Secondary Antibodies

     

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    Bioinformatics

    Gene Symbol EPHA3
    Uniprot