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Epac2 Antibody

Images

 
Western Blot: Epac2 Antibody [NBP2-32009] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: Epac2 Antibody [NBP2-32009] - Staining of human cell line RT4 shows localization to focal adhesion sites. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Epac2 Antibody [NBP2-32009] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Epac2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: VIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQGNSQPQQKYTVMSGTPEKILEHFLETIRLEATLNEATDSVLNDFIMMHCVFMPNTQLC
Predicted Species
Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAPGEF4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Epac2 Protein (NBP2-32009PEP)
Reviewed Applications
Read 1 Review rated 3
using
NBP2-32009 in the following applications:

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Epac2 Antibody

  • cAMP-GEFII
  • cAMP-regulated guanine nucleotide exchange factor II
  • CGEF2
  • CGEF2EPAC 2
  • EPAC 2
  • Epac2
  • Exchange factor directly activated by cAMP 2
  • Exchange protein directly activated by cAMP 2
  • Nbla00496
  • putative protein product of Nbla00496
  • Rap guanine nucleotide exchange factor (GEF) 4
  • rap guanine nucleotide exchange factor 4
  • RAP guanine-nucleotide-exchange factor (GEF) 4
  • RAPGEF4

Background

Epac2, also known as Rap Guanine Nucleotide Exchange Factor (GEF) 4, is a guanine nucleotide exchange factor for RAP1A, RAP1B and RAP2A small GTPases. Studies have shown that EPAC2 has a significant role in the development of type 2 diabetes. EPAC2 has also been studied in relation to ataxia, neuronitis, osteoporosis and pancreatitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Epac2 Antibody (NBP2-32009) (0)

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Review for Epac2 Antibody (NBP2-32009) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-32009:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot Epac2 NBP2-32009
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3
reviewed by:
Verified Customer
WB Mouse 04/25/2019
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Summary

ApplicationWestern Blot
Sample TestedMin6 mouse insulinoma whole cell lysate
SpeciesMouse
LotR31753

Comments

CommentsWestern Blot: Epac2 Ab [NBP2-32009] - Analysis of MIN6 cell lysates. Lane 1: Molecular Weight Ladder. Lanes 2-5: MIN6 cells (~10 ug). Primary Antibody: Anti-Epac2 Ab at 1:750 overnight at 4C. Secondary Antibody: Goat Anti-rabbit IgG: HRP at 1:3000 for 1hr RT.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Epac2 Antibody (NBP2-32009) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Epac2 Products

Research Areas for Epac2 Antibody (NBP2-32009)

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Blogs on Epac2

There are no specific blogs for Epac2, but you can read our latest blog posts.
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Recent Reviews

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Verified Customer
04/25/2019
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol RAPGEF4
Uniprot