![Western Blot: EMSY Antibody [NB100-545] - Detection of Human EMSY by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/EMSY-Antibody-Western-Blot-NB100-545-img0013.jpg "Western Blot: EMSY Antibody [NB100-545] - Detection of Human EMSY by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1250 and the C-terminus (residue 1322) of human EMSY chromosome 11 open reading frame 30 using the numbering given in TrEMBL entry Q8TE50 (GeneID 56946). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | C11ORF30 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for EMSY Antibody (NB100-545)Find related products by research area.
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![Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545] - Section of human prostate carcinoma. Affinity purified rabbit anti-Emsy used at a dilution of 1:100 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EMSY-Antibody-Immunohistochemistry-Paraffin-NB100-545-img0015.jpg "Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545] - Section of human prostate carcinoma. Affinity purified rabbit anti-Emsy used at a dilution of 1:100 (1 ug/ml). Detection: DAB")
![Western Blot: EMSY Antibody [NB100-545] - Nuclear extract (NE) from HeLa cells.](http://images.novusbio.com/fullsize/EMSY-Antibody-Western-Blot-NB100-545-img0010.jpg "Western Blot: EMSY Antibody [NB100-545] - Nuclear extract (NE) from HeLa cells.")
![Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-EMSY used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EMSY-Antibody-Immunohistochemistry-Paraffin-NB100-545-img0011.jpg "Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-EMSY used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Immunoprecipitation: EMSY Antibody [NB100-545] - Detection of human EMSY by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 (lot NB100-545-2) used for IP at 6 ug/mg lysate. EMSY was also immunoprecipitated by a previous lot of this antibody (lot NB100-545-1) and another rabbit anti-EMSY antibody. For blotting immunoprecipitated EMSY, NB100-545 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/EMSY-Antibody-Immunoprecipitation-NB100-545-img0014.jpg "Immunoprecipitation: EMSY Antibody [NB100-545] - Detection of human EMSY by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 (lot NB100-545-2) used for IP at 6 ug/mg lysate. EMSY was also immunoprecipitated by a previous lot of this antibody (lot NB100-545-1) and another rabbit anti-EMSY antibody. For blotting immunoprecipitated EMSY, NB100-545 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")