![Western Blot: EMP2 Overexpression Lysate (Native) [NBL1-10260] - analysis of EMP2 in EMP2 Overexpression Lysate using anti-EMP2 antibody (Cat.# NBP1-86847, 1:500 dilution). Image from verified customer review.](http://images.novusbio.com/fullsize/EMP2-Overexpression-Lysate-Native-Western-Blot-NBL1-10260-img0003.jpg "Western Blot: EMP2 Overexpression Lysate (Native) [NBL1-10260] - analysis of EMP2 in EMP2 Overexpression Lysate using anti-EMP2 antibody (Cat.# NBP1-86847, 1:500 dilution). Image from verified customer review.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Description | EMP2 Transient Overexpression LysateExpression Host: HEK293T Plasmid: RC201995 Accession#: NM_001424 Protein Tag: C-MYC/DDK You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT. |
| Gene | EMP2 |
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| Application Notes | This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (cat# NBP1-71705) present on the protein construct. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading. |
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| Theoretical MW | 19 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | RIPA buffer |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
WB | Mouse | 01/26/2015 |
Summary
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Research Areas for EMP2 Lysate (NBL1-10260)Find related products by research area.
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| Gene Symbol | EMP2 |
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