![Western Blot: EMI1 Antibody (EMI1/1176) [NBP2-44344] - Western Blot of HeLa, HepG2, HEK293 and K562 cell lysates with EMI1 Antibody (EMI1/1176).](http://images.novusbio.com/fullsize/EMI1-Antibody-EMI1-1176-Western-Blot-NBP2-44344-img0008.jpg "Western Blot: EMI1 Antibody (EMI1/1176) [NBP2-44344] - Western Blot of HeLa, HepG2, HEK293 and K562 cell lysates with EMI1 Antibody (EMI1/1176).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, MA |
| Clone | EMI1/1176 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47786) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein (Uniprot: Q9UKT4) |
| Localization | Nuclear |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FBXO5 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. Western Blot: 1-2ug/ml for 60 minutes at RT. |
| Theoretical MW | 56 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg)
![Immunohistochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunohistochemistry_15886.jpg)
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_16572.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) [NBP2-44344] - Human Ovarian carcinoma stained with EMI1 Monoclonal Antibody (EMI1/1176).](http://images.novusbio.com/fullsize/EMI1-Antibody-EMI1-1176-Immunohistochemistry-Paraffin-NBP2-44344-img0005.jpg "Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) [NBP2-44344] - Human Ovarian carcinoma stained with EMI1 Monoclonal Antibody (EMI1/1176).")
![Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) [NBP2-44344] - Human Ovarian Carcinoma stained with EMI1 Monoclonal Antibody (EMI1/1176).](http://images.novusbio.com/fullsize/EMI1-Antibody-EMI1-1176-Immunohistochemistry-Paraffin-NBP2-44344-img0004.jpg "Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) [NBP2-44344] - Human Ovarian Carcinoma stained with EMI1 Monoclonal Antibody (EMI1/1176).")
![Protein Array: EMI1 Antibody (EMI1/1176) [NBP2-44344] - Analysis of Protein Array containing more than 19,000 full-length human proteins using EMI1 Antibody (EMI1/1176). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/EMI1-Antibody-EMI1-1176-Protein-Array-NBP2-44344-img0007.jpg "Protein Array: EMI1 Antibody (EMI1/1176) [NBP2-44344] - Analysis of Protein Array containing more than 19,000 full-length human proteins using EMI1 Antibody (EMI1/1176). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.")
![Bioactivity GM-CSF [Unconjugated]](https://images.novusbio.com/images/protein/7954-gmcf_recombinant-human-gm-csf-cho-expressed-protein-cf-bioactivity-272020121514.jpg)
