![Western Blot: eIF4GII Antibody [NBP2-16309] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with eIF4GII antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/eIF4GII-Antibody-Western-Blot-NBP2-16309-img0016.jpg "Western Blot: eIF4GII Antibody [NBP2-16309] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with eIF4GII antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human eIF4GII. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EIF4G3 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 177 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Neutralization CXCL8/IL-8 Antibody (6217) [Unconjugated]](https://images.novusbio.com/images2/CXCL8_MAB208_Block_Neutralize_9064.jpg)
![Immunocytochemistry CXCL8/IL-8 Antibody (6217) [Unconjugated]](https://images.novusbio.com/images2/CXCL8_MAB208_Immunocytochemistry_9339.jpg)
![Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]](https://images.novusbio.com/images2/mab208_human-il-8-cxcl8-mab-clone-6217-western-blot-1882021143847.jpg)
| Publication using NBP2-16309 | Applications | Species |
|---|---|---|
| Dai P, Wang X, Gou LT, et al. A Translation-Activating Function of MIWI/piRNA during Mouse Spermiogenesis Cell 2019-12-12 [PMID: 31835033] |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
LEI ZHANG |
WB | Mouse | 04/02/2018 |
Summary
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - eIF4GII antibody detects eIF4GII protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. eIF4GII stained by eIF4GII antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/eIF4GII-Antibody-Immunohistochemistry-Paraffin-NBP2-16309-img0019.jpg "Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - eIF4GII antibody detects eIF4GII protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. eIF4GII stained by eIF4GII antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: eIF4GII Antibody [NBP2-16309] - A: Marker. B: mouse testes. 6% SDS-PAGE gel, antibody diluted 1:10000. This image was submitted via customer review.](http://images.novusbio.com/fullsize/eIF4GII-Antibody-Western-Blot-NBP2-16309-img0013.jpg "Western Blot: eIF4GII Antibody [NBP2-16309] - A: Marker. B: mouse testes. 6% SDS-PAGE gel, antibody diluted 1:10000. This image was submitted via customer review.")
![Western Blot: eIF4GII Antibody [NBP2-16309] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with eIF4GII antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/eIF4GII-Antibody-Western-Blot-NBP2-16309-img0015.jpg "Western Blot: eIF4GII Antibody [NBP2-16309] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with eIF4GII antibody diluted at 1:10000.")
![Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - Paraffin-embedded mouse cervix. EIF4G3 antibody [N1], N-term dilution: 1:500.](http://images.novusbio.com/fullsize/eIF4GII-Antibody-Immunohistochemistry-Paraffin-NBP2-16309-img0005.jpg "Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - Paraffin-embedded mouse cervix. EIF4G3 antibody [N1], N-term dilution: 1:500.")
![Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - Paraffin-embedded rat hind brain. EIF4G3 antibody [N1], N-term dilution: 1:500.](http://images.novusbio.com/fullsize/eIF4GII-Antibody-Immunohistochemistry-Paraffin-NBP2-16309-img0006.jpg "Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - Paraffin-embedded rat hind brain. EIF4G3 antibody [N1], N-term dilution: 1:500.")
![Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - Paraffin-embedded mouse brain stem. EIF4G3 antibody [N1], N-term dilution: 1:500.](http://images.novusbio.com/fullsize/eIF4GII-Antibody-Immunohistochemistry-Paraffin-NBP2-16309-img0011.jpg "Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - Paraffin-embedded mouse brain stem. EIF4G3 antibody [N1], N-term dilution: 1:500.")
![Immunohistochemistry-Frozen: eIF4GII Antibody [NBP2-16309] - eIF4GII antibody detects eIF4GII protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: eIF4GII protein stained by eIF4GII antibody diluted at 1:250. Red: NeuN, stained by NeuN antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/eIF4GII-Antibody-Immunohistochemistry-Frozen-NBP2-16309-img0014.jpg "Immunohistochemistry-Frozen: eIF4GII Antibody [NBP2-16309] - eIF4GII antibody detects eIF4GII protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: eIF4GII protein stained by eIF4GII antibody diluted at 1:250. Red: NeuN, stained by NeuN antibody diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - eIF4GII antibody [N1], N-term detects eIF4GII protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. eIF4GII stained by eIF4GII diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/eIF4GII-Antibody-Immunohistochemistry-Paraffin-NBP2-16309-img0017.jpg "Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - eIF4GII antibody [N1], N-term detects eIF4GII protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. eIF4GII stained by eIF4GII diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - eIF4GII antibody detects eIF4GII protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. eIF4GII stained by eIF4GII antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/eIF4GII-Antibody-Immunohistochemistry-Paraffin-NBP2-16309-img0018.jpg "Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - eIF4GII antibody detects eIF4GII protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. eIF4GII stained by eIF4GII antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunoprecipitation: eIF4GII Antibody [NBP2-16309] - Immunoprecipitation of EIF4G3 protein from 293T whole cell extracts using 5 ug of EIF4G3 antibody [N1], N-term. Western blot analysis was performed using EIF4G3 ntibody [N1], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent](http://images.novusbio.com/fullsize/eIF4GII-Antibody-Immunoprecipitation-NBP2-16309-img0010.jpg "Immunoprecipitation: eIF4GII Antibody [NBP2-16309] - Immunoprecipitation of EIF4G3 protein from 293T whole cell extracts using 5 ug of EIF4G3 antibody [N1], N-term. Western blot analysis was performed using EIF4G3 ntibody [N1], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent")
![eIF4GII Antibody [NBP2-16309] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF4G3 protein stained by EIF4G3 antibody [N1], N-term diluted at 1:500. Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/antibody/nbp2-16309_rabbit-polyclonal-eif4gii-antibody-immunocytochemistry-immunofluorescence-175202372326.jpg "eIF4GII Antibody [NBP2-16309] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF4G3 protein stained by EIF4G3 antibody [N1], N-term diluted at 1:500. Blue: Hoechst 33342 staining.Scale bar = 10 um.")
![Western Blot eIF4E [p Ser209] Antibody](https://images.novusbio.com/images/eIF4E-[p-Ser209]-Antibody-Western-Blot-NB100-81898-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence eIF4E [p Ser209] Antibody](https://images.novusbio.com/images/eIF4E-[p-Ser209]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81898-img0005.jpg)
![Immunohistochemistry-Paraffin eIF4E [p Ser209] Antibody](https://images.novusbio.com/images/eIF4E-[p-Ser209]-Antibody-Immunohistochemistry-NB100-81898-img0006.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
