Is the His-tag on #NBP1-78833 EIF4A3 protein on the n-term or the c-term?

Name: Recombinant Human EIF4A3 His Protein
Brand: Novus Biologicals
SKU: NBP1-78833
Price: 329 USD

Question

Accepted Answer

The His tag on this protein is located on the N-terminus.

Description	A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-411 of Human EIF4A3 Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMATTAT MATSGSARKR LLKEEDMTKV EFETSEEVDV TPTFDTMGLR EDLLRGIYAY GFEKPSAIQQ RAIKQIIKGR DVIAQSQSGT GKTATFSISV LQCLDIQVRE TQALILAPTR ELAVQIQKGL LALGDYMNVQ CHACIGGTNV GEDIRKLDYG QHVVAGTPGR VFDMIRRRSL RTRAIKMLVL DEADEMLNKG FKEQIYDVYR YLPPATQVVL ISATLPHEIL EMTNKFMTDP IRILVKRDEL TLEGIKQFFV AVEREEWKFD TLCDLYDTLT ITQAVIFCNT KRKVDWLTEK MREANFTVSS MHGDMPQKER ESIMKEFRSG ASRVLISTDV WARGLDVPQV SLIINYDLPN NRELYIHRIG RSGRYGRKGV AINFVKNDDI RILRDIEQYY STQIDEMPMN VADLI
Source	E. coli
Protein/Peptide Type	Recombinant Protein
Gene	EIF4A3
Purity	>95%, by SDS-PAGE

Dilutions	SDS-Page
Theoretical MW	49.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 30% glycerol, 200 mM NaCl
Preservative	No Preservative
Concentration	0.5 mg/ml
Purity	>95%, by SDS-PAGE

Recombinant Human EIF4A3 His Protein