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eIF4A2 Antibody (PCRP-EIF4A2-2B5) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, MA
Clone
PCRP-EIF4A2-2B5
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

eIF4A2 Antibody (PCRP-EIF4A2-2B5) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Recombinant full-length human eIF4A2 protein (Uniprot: Q14240)
Localization
Nucleus.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
EIF4A2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Protein Array
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Predicted to work in Chick, Mouse, Rat, Xenopus and Zebrafish.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for eIF4A2 Antibody (PCRP-EIF4A2-2B5) [CoraFluor™ 1]

  • ATP-dependent RNA helicase eIF4A-2
  • BM-010
  • DDX2Beukaryotic translation initiation factor 4A, isoform 2
  • EC 3.6.1
  • EC 3.6.4.13
  • EIF4A
  • eIF-4A-II
  • eIF4A-II
  • EIF4F
  • eukaryotic initiation factor 4A-II
  • eukaryotic translation initiation factor 4A
  • eukaryotic translation initiation factor 4A2

Background

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and isrequired for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondarystructures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, andsubsequent scanning for the initiator codon

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol EIF4A2