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EIF2B2 Antibody (5B12-E10)

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Western Blot: EIF2B2 Antibody (5B12-E10) [H00008892-M01] - EIF2B2 monoclonal antibody (M01), clone 5B12-E10 Analysis of EIF2B2 expression in K-562.
Immunohistochemistry-Paraffin: EIF2B2 Antibody (5B12-E10) [H00008892-M01] - Analysis of monoclonal antibody to EIF2B2 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 5 ug/ml.
Western Blot: EIF2B2 Antibody (5B12-E10) [H00008892-M01] - EIF2B2 monoclonal antibody (M01), clone 5B12-E10. Analysis of EIF2B2 expression in HeLa.
Western Blot: EIF2B2 Antibody (5B12-E10) [H00008892-M01] - Analysis of EIF2B2 expression in transfected 293T cell line by EIF2B2 monoclonal antibody (M01), clone 5B12-E10.Lane 1: EIF2B2 transfected lysate(39 KDa).Lane ...read more
Sandwich ELISA: EIF2B2 Antibody (5B12-E10) [H00008892-M01] - Detection limit for recombinant GST tagged EIF2B2 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
5B12-E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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EIF2B2 Antibody (5B12-E10) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
EIF2B2 (AAH00494, 1 a.a. ~ 351 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPGSAAKGSELSERIESFVETLKRGGGPRSSEEMARETLGLLRQIITDHRWSNAGELMELIRREGRRMTAAQPSETTVGNMVRRVLKIIREEYGRLHGRTDESDQQESLHKLLTSGGLNEDFSFHYAQLQSNIIEAINELLVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKEAARKRKFHVIVAECAPFCQGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGALRAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDSFHKFVAPEEVLPFTEGDILEKVSVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYHPDDHVL
Specificity
EIF2B2 - eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EIF2B2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EIF2B2 Antibody (5B12-E10)

  • eIF-2B GDP-GTP exchange factor subunit beta
  • EIF2B
  • EIF2BB
  • EIF-2Bbeta
  • eukaryotic translation initiation factor 2B, subunit 2 (beta, 39kD)
  • eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa
  • S20I15
  • S20III15
  • translation initiation factor eIF-2B subunit beta

Background

Eukaryotic initiation factor-2B (EIF2B) is a GTP exchange protein essential for protein synthesis. It consists of alpha (EIF2B1; MIM 606686), beta (EIF2B2), gamma (EIF2B3; MIM 606273), delta (EIF2B4; MIM 606687), and epsilon (EIF2B5; MIM 603945) subunits. EIF2B activates its EIF2 (see MIM 603907) substrate by exchanging EIF2-bound GDP for GTP.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol EIF2B2
Entrez
OMIM
Uniprot