![Western Blot: eIF2 alpha/EIF2S1 Antibody (OTI3H4) - Azide and BSA Free [NBP2-70626] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eIF2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-eIF2A.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-OTI3H4-Azide-and-BSA-Free-Western-Blot-NBP2-70626-img0002.jpg "Western Blot: eIF2 alpha/EIF2S1 Antibody (OTI3H4) - Azide and BSA Free [NBP2-70626] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eIF2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-eIF2A.")
| Reactivity | Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI3H4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human EIF2S1 (NP_004085) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | EIF2S1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 35.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
![Western Blot PKLR Antibody [Unconjugated]](https://images.novusbio.com/images2/PKLR_AF8519_Western_Blot_16592.jpg)
![Simple Western PKLR Antibody [Unconjugated]](https://images.novusbio.com/images2/af8519_human-mouse-rat-pklr-affinity-purified-pab-simple-western-1342022154119.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | EIF2S1 |
![Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 Antibody (OTI3H4) - Azide and BSA Free [NBP2-70626] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY eIF2A.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-OTI3H4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70626-img0004.jpg "Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 Antibody (OTI3H4) - Azide and BSA Free [NBP2-70626] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY eIF2A.")
![Immunohistochemistry: eIF2 alpha/EIF2S1 Antibody (OTI3H4) - Azide and BSA Free [NBP2-70626] - Staining of paraffin-embedded Human pancreas tissue using anti-eIF2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-OTI3H4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70626-img0008.jpg "Immunohistochemistry: eIF2 alpha/EIF2S1 Antibody (OTI3H4) - Azide and BSA Free [NBP2-70626] - Staining of paraffin-embedded Human pancreas tissue using anti-eIF2A mouse monoclonal antibody.")
![Flow Cytometry: eIF2 alpha/EIF2S1 Antibody (OTI3H4) - Azide and BSA Free [NBP2-70626] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-eIF2A antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-OTI3H4-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70626-img0003.jpg "Flow Cytometry: eIF2 alpha/EIF2S1 Antibody (OTI3H4) - Azide and BSA Free [NBP2-70626] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-eIF2A antibody, and then analyzed by flow cytometry.")
![Western Blot: eIF2 alpha/EIF2S1 Antibody (OTI3H4) - Azide and BSA Free [NBP2-70626] - Analysis of extracts (35ug) from 9 different cell lines by using anti-eIF2A monoclonal antibody.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-OTI3H4-Azide-and-BSA-Free-Western-Blot-NBP2-70626-img0001.jpg "Western Blot: eIF2 alpha/EIF2S1 Antibody (OTI3H4) - Azide and BSA Free [NBP2-70626] - Analysis of extracts (35ug) from 9 different cell lines by using anti-eIF2A monoclonal antibody.")
![Immunohistochemistry: eIF2 alpha/EIF2S1 Antibody (OTI3H4) - Azide and BSA Free [NBP2-70626] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-eIF2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-OTI3H4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70626-img0005.jpg "Immunohistochemistry: eIF2 alpha/EIF2S1 Antibody (OTI3H4) - Azide and BSA Free [NBP2-70626] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-eIF2A mouse monoclonal antibody.")
![Immunohistochemistry: eIF2 alpha/EIF2S1 Antibody (OTI3H4) - Azide and BSA Free [NBP2-70626] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-eIF2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-OTI3H4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70626-img0006.jpg "Immunohistochemistry: eIF2 alpha/EIF2S1 Antibody (OTI3H4) - Azide and BSA Free [NBP2-70626] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-eIF2A mouse monoclonal antibody.")
![Immunohistochemistry: eIF2 alpha/EIF2S1 Antibody (OTI3H4) - Azide and BSA Free [NBP2-70626] - Staining of paraffin-embedded Human Kidney tissue using anti-eIF2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-OTI3H4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70626-img0007.jpg "Immunohistochemistry: eIF2 alpha/EIF2S1 Antibody (OTI3H4) - Azide and BSA Free [NBP2-70626] - Staining of paraffin-embedded Human Kidney tissue using anti-eIF2A mouse monoclonal antibody.")
![Immunohistochemistry: eIF2 alpha/EIF2S1 Antibody (OTI3H4) - Azide and BSA Free [NBP2-70626] - Staining of paraffin-embedded Human prostate tissue using anti-eIF2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-OTI3H4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70626-img0009.jpg "Immunohistochemistry: eIF2 alpha/EIF2S1 Antibody (OTI3H4) - Azide and BSA Free [NBP2-70626] - Staining of paraffin-embedded Human prostate tissue using anti-eIF2A mouse monoclonal antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")