![Western Blot: EHD3 Antibody (4B7) [H00030845-M01] - EHD3 monoclonal antibody (M01), clone 4B7 Analysis of EHD3 expression in IMR-32.](http://images.novusbio.com/fullsize/EHD3-Antibody-4B7-Western-Blot-H00030845-M01-img0013.jpg "Western Blot: EHD3 Antibody (4B7) [H00030845-M01] - EHD3 monoclonal antibody (M01), clone 4B7 Analysis of EHD3 expression in IMR-32.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 4B7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | EHD3 (NP_055415, 357 a.a. ~ 406 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KRMQDQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEESQRPI |
| Specificity | EHD3 - EH-domain containing 3 |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | EHD3 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA. |
|
| Publications |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
![Western Blot EPS15 Antibody [Unconjugated]](https://images.novusbio.com/images2/Eps15_AF8480_Western_Blot_16272.jpg)
![Immunocytochemistry EPS15 Antibody [Unconjugated]](https://images.novusbio.com/images2/Eps15_AF8480_Immunocytochemistry_16281.jpg)
![Immunocytochemistry EPS15 Antibody [Unconjugated]](https://images.novusbio.com/images2/Eps15_AF8480_Immunocytochemistry_16416.jpg)
![Immunocytochemistry Prohibitin Antibody [Unconjugated]](https://images.novusbio.com/images2/Prohibitin_AF3470_Immunocytochemistry__Immunofluorescence_20123.jpg)
| Publication using H00030845-M01 | Applications | Species |
|---|---|---|
| Dumas A, Le-Bury G, Marie-Anais F et al. The HIV-1 protein Vpr impairs phagosome maturation by controlling microtubule-dependent trafficking. J Cell Biol. 2015-10-26 [PMID: 26504171] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: EHD3 Antibody (4B7) [H00030845-M01] - Analysis of monoclonal antibody to EHD3 on HeLa cell. Antibody concentration 25 ug/ml.](http://images.novusbio.com/fullsize/EHD3-Antibody-4B7-Immunocytochemistry-Immunofluorescence-H00030845-M01-img0008.jpg "Immunocytochemistry/Immunofluorescence: EHD3 Antibody (4B7) [H00030845-M01] - Analysis of monoclonal antibody to EHD3 on HeLa cell. Antibody concentration 25 ug/ml.")
![Immunohistochemistry-Paraffin: EHD3 Antibody (4B7) [H00030845-M01] - Analysis of monoclonal antibody to EHD3 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/EHD3-Antibody-4B7-Immunohistochemistry-Paraffin-H00030845-M01-img0010.jpg "Immunohistochemistry-Paraffin: EHD3 Antibody (4B7) [H00030845-M01] - Analysis of monoclonal antibody to EHD3 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.")
![Western Blot: EHD3 Antibody (4B7) [H00030845-M01] - EHD3 monoclonal antibody (M01), clone 4B7. Analysis of EHD3 expression in COLO 320 HSR.](http://images.novusbio.com/fullsize/EHD3-Antibody-4B7-Western-Blot-H00030845-M01-img0012.jpg "Western Blot: EHD3 Antibody (4B7) [H00030845-M01] - EHD3 monoclonal antibody (M01), clone 4B7. Analysis of EHD3 expression in COLO 320 HSR.")
![Western Blot: EHD3 Antibody (4B7) [H00030845-M01] - EHD3 monoclonal antibody (M01), clone 4B7. Analysis of EHD3 expression in MCF-7.](http://images.novusbio.com/fullsize/EHD3-Antibody-4B7-Western-Blot-H00030845-M01-img0014.jpg "Western Blot: EHD3 Antibody (4B7) [H00030845-M01] - EHD3 monoclonal antibody (M01), clone 4B7. Analysis of EHD3 expression in MCF-7.")
![ELISA: EHD3 Antibody (4B7) [H00030845-M01] - Detection limit for recombinant GST tagged EHD3 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/EHD3-Antibody-4B7-ELISA-H00030845-M01-img0015.jpg "ELISA: EHD3 Antibody (4B7) [H00030845-M01] - Detection limit for recombinant GST tagged EHD3 is approximately 0.1ng/ml as a capture antibody.")
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![Immunocytochemistry Nestin Antibody (196908) [Unconjugated]](https://images.novusbio.com/images2/Nestin_MAB1259_Immunocytochemistry_6561.jpg)
![Intracellular Staining by Flow Cytometry Nestin Antibody (196908) [Unconjugated]](https://images.novusbio.com/images/antibody/mab1259_human-nestin-mab-clone-196908-intracellular-staining-by-flow-cytometry-24120239959.jpg)
![Western Blot Nestin Antibody (196908) [Unconjugated]](https://images.novusbio.com/images/mab1259_human-nestin-mab-clone-196908-41202410481129.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")