![Western Blot: EDEM2 Antibody (2E4) [H00055741-M01] - Analysis of EDEM2 expression in transfected 293T cell line by C20orf31 monoclonal antibody (M01), clone 2E4.Lane 1: EDEM2 transfected lysate(64.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/EDEM2-Antibody-2E4-Western-Blot-H00055741-M01-img0007.jpg "Western Blot: EDEM2 Antibody (2E4) [H00055741-M01] - Analysis of EDEM2 expression in transfected 293T cell line by C20orf31 monoclonal antibody (M01), clone 2E4.Lane 1: EDEM2 transfected lysate(64.8 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC, IP |
| Clone | 2E4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | C20orf31 (NP_060687, 479 a.a. ~ 578 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PIDPAALHCCQRLKEEQWEVEDLMREFYSLKRCRSKFQKNTVSSGPWEPPARPGTLFSPENHDQARERKPAKQKVPLLSCPSQPFTSKLALLGQVFLDSS |
| Specificity | C20orf31 - chromosome 20 open reading frame 31 |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | EDEM2 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: EDEM2 Antibody (2E4) [H00055741-M01] - Analysis of monoclonal antibody to C20orf31 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/EDEM2-Antibody-2E4-Immunohistochemistry-Paraffin-H00055741-M01-img0004.jpg "Immunohistochemistry-Paraffin: EDEM2 Antibody (2E4) [H00055741-M01] - Analysis of monoclonal antibody to C20orf31 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1.5 ug/ml.")
![ELISA: EDEM2 Antibody (2E4) [H00055741-M01] - Detection limit for recombinant GST tagged C20orf31 is approximately 30ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/EDEM2-Antibody-2E4-ELISA-H00055741-M01-img0002.jpg "ELISA: EDEM2 Antibody (2E4) [H00055741-M01] - Detection limit for recombinant GST tagged C20orf31 is approximately 30ng/ml as a capture antibody.")
![Immunoprecipitation: EDEM2 Antibody (2E4) [H00055741-M01] - Analysis of EDEM2 transfected lysate using anti-EDEM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EDEM2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/EDEM2-Antibody-2E4-Immunoprecipitation-H00055741-M01-img0005.jpg "Immunoprecipitation: EDEM2 Antibody (2E4) [H00055741-M01] - Analysis of EDEM2 transfected lysate using anti-EDEM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EDEM2 MaxPab rabbit polyclonal antibody.")
![Sandwich ELISA: EDEM2 Antibody (2E4) [H00055741-M01] - Detection limit for recombinant GST tagged EDEM2 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/EDEM2-Antibody-2E4-Sandwich-ELISA-H00055741-M01-img0006.jpg "Sandwich ELISA: EDEM2 Antibody (2E4) [H00055741-M01] - Detection limit for recombinant GST tagged EDEM2 is 0.1 ng/ml as a capture antibody.")
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