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DSCAM Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: DSCAM Antibody [NBP2-30716] - Staining in human cerebral cortex and skeletal muscle tissues.. Corresponding DSCAM RNA-seq data are presented for the same ...read more
Immunocytochemistry/ Immunofluorescence: DSCAM Antibody [NBP2-30716] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: DSCAM Antibody [NBP2-30716] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: DSCAM Antibody [NBP2-30716] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: DSCAM Antibody [NBP2-30716] - Staining of human cerebellum shows strong cytoplasmic positivity in cells in granular layer.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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DSCAM Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: PRAQLLIEERDTMETIDDRSTVLLTDADFGEAAKQKSLTVTHTVHYQSVSQATGPLVDVSDARPGTNPTTRRNAKAGPTARNRYASQWTLNRPHPTISAHTLTTDWRLPTPRAAGSVDKESDSYSVSPSQDTDRARSS
Predicted Species
Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DSCAM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DSCAM Protein (NBP2-30716PEP)
Publications
Read Publication using NBP2-30716.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DSCAM Antibody

  • CHD2-42
  • CHD2-52
  • Down syndrome cell adhesion molecule
  • DSCAM
  • human CHD2-52 down syndrome cell adhesion molecule, 10CHD2

Background

The Dscam gene encodes a large family of axon guidance receptors and immune receptors via alternative splicing, generating over 38000 isoforms. The gene has been shown to be required for axon guidance, specifically targeting individual neurons to their targets. The gene has also been shown to be involved in the immune system of the fly; different isoforms interact with different pathogens to elicit an immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DSCAM Antibody (NBP2-30716)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for DSCAM Antibody (NBP2-30716) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on DSCAM.

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Bioinformatics

Gene Symbol DSCAM
Uniprot