![Western Blot: DOK1 [p Ser450] Antibody [NBP3-23286] - Western blot image of Jurkat cells stimulated with calyculin A (100 nM, 30 min) (lanes 1-6) followed by lambda phosphatase (lanes 2 & 6) or alkaline phosphatase (lane 4) treatment. The blots were probed with anti-Dok1 (lanes 1 & 2), anti-Dok1 (Tyr-362) (lanes 3 & 4), and anti-Dok1 (Ser-450) (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23286_rabbit-dok1-p-ser450-pab-1992023141012.jpg "Western Blot: DOK1 [p Ser450] Antibody [NBP3-23286] - Western blot image of Jurkat cells stimulated with calyculin A (100 nM, 30 min) (lanes 1-6) followed by lambda phosphatase (lanes 2 & 6) or alkaline phosphatase (lane 4) treatment. The blots were probed with anti-Dok1 (lanes 1 & 2), anti-Dok1 (Tyr-362) (lanes 3 & 4), and anti-Dok1 (Ser-450) (lanes 5 & 6).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Stable for at least 1 year at -20°C. |
| Immunogen | Phospho-Dok1 (Ser-450) synthetic peptide (coupled to KLH) corresponds to amino acids surrounding serine 450 in human Dok1. This sequence is conserved in Dok1 from rat (Ser-449) and mouse (Ser-451). The site is not conserved in other Dok family members. [UniProt# Q99704] |
| Modification | p Ser450 |
| Specificity | This antibody was cross-adsorbed to an unphosphorylated Dok1 (Ser-450) peptide before affinity purification using phospho-Dok1 (Ser-450) peptide. The purified antibody detects a band at 62 kDa corresponding to Dok1 in western blots of human Jurkat cells, but does not detect this band after lambda phosphatase treatment. Similar to Dok1 (Tyr-362) antibody, this antibody also detects an 80 kDa band that has not been identified. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DOK1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 62 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. |
| Buffer | PBS, 1 mg/ml BSA and 50% glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Antigen Affinity-purified |
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![DOK1 [p Ser450] Antibody](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "DOK1 [p Ser450] Antibody")
Secondary Antibodies |
Isotype Controls |
Research Areas for DOK1 Antibody (NBP3-23286)Find related products by research area.
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| Gene Symbol | DOK1 |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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