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DLX5 Antibody (712216)

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DLX5 was detected in immersion fixed human mesenchymal stem cells differentiated into osteocytes using Mouse Anti-Human DLX5 Monoclonal Antibody (Catalog # MAB6710) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
DLX5 was detected in immersion fixed human mesenchymal stem cells differentiated into osteocytes using Mouse Anti-Human DLX5 Monoclonal Antibody (Catalog # MAB6710) at 10 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clone
712216
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6710-SP
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DLX5 Antibody (712216) Summary

Immunogen
E. coli-derived recombinant human DLX5
Asn216-Tyr289
Accession # P56178
Specificity
Detects human DLX5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human DLX4 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
DLX5
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DLX5 Antibody (712216)

  • distal-less homeo box 5
  • distal-less homeobox 5
  • DLX5
  • homeobox protein DLX-5

Background

DLX5 (distal-less homeobox 5) is a 33-35 kDa member of the distal-less homeobox family of transcription factors. It is expressed in embryonic mesenchyme where it promotes cartilage and bone formation, and in nervous tissue where it promotes interneuron development. Human DLX5 is 289 amino acids (aa) in length. It contains one DNA-binding homeobox domain (aa 137-196) and two phosphorylation sites at Ser34 and Ser217 that, when utilized, increases transcriptional activity. DLX5 likely forms homodimers, and is known to heterodimerize with MSX-2. Over aa 216-289, human DLX5 shares 99% aa identity with mouse DLX5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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DLX5 Antibody
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DLX5 Antibody (712216)
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Bioinformatics

Gene Symbol DLX5
Uniprot