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Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Staining in human kidney and skin tissues using anti-DDAH1 antibody. Corresponding ...read more
Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol.
Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Analysis in human cell line SH-SY5Y.
Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Staining of human kidney shows high expression.
Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Staining of human skin shows low expression as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SKRTNQRGAEILADTFKDYAVSTVPVADGLHLKSFCSMAGPNLIAIGSSESAQKALKIMQQMSDHRYDKLTVPDDIAANCIYLNIPNKGHVLLHRTPEEYPESAKVYEKLKDHMLIPVSMSELEKVDGLLT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDAH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 22440215). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Protein (NBP1-85273PEP)
Publications
Read Publication using
NBP1-85273 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 22440215).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

  • DDAH1
  • DDAH-1
  • DDAHdimethylargininase-1
  • DDAHI
  • Dimethylargininase-1
  • dimethylarginine dimethylaminohydrolase 1FLJ25539
  • EC 3.5.3.18
  • FLJ21264
  • N(G)
  • N(G)-dimethylarginine dimethylaminohydrolase 1
  • NG, NG-dimethylarginine dimethylaminohydrolase

Background

DDAH1 belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody (NBP1-85273)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IF/IHC.


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IF/IHC
(1)
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Human
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DDAH1