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DHX8 Antibody

Images

 
Western Blot: DHX8 Antibody [NB100-1713] - Detection of human DHX8 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified ...read more
Immunohistochemistry: DHX8 Antibody [NB100-1713] - Detection of human DHX8 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody:Affinity purified rabbit anti-DHX8 antibody (NB100-1713).
Immunohistochemistry-Paraffin: DHX8 Antibody [NB100-1713] - Human ovarian carcinoma (left) and mouse teratoma (right). Antibody used at a dilution of 1:200 (1ug/ml).
Immunohistochemistry: DHX8 Antibody [NB100-1713] - Detection of mouse DHX8 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody:Affinity purified rabbit anti-DHX8 antibody (NB100-1713).
Immunoprecipitation: DHX8 Antibody [NB100-1713] - Detection of human DHX8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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DHX8 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 325 and 375 of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 using the numbering given in entry NP_004932.1 (GeneID 1659).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DHX8
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DHX8 Antibody

  • DDX8ATP-dependent RNA helicase DHX8
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase)
  • DEAH (Asp-Glu-Ala-His) box polypeptide 8
  • DEAH box protein 8
  • DEAH-box protein 8
  • EC 3.6.1
  • EC 3.6.4.13
  • HRH1RNA helicase HRH1
  • PRP22
  • PRPF22

Background

DHX8 is a member of the DEAH box family of proteins that possesses several conserved motifs which include the highly conserved DEAH (Asp-Glu-Ala-His) amino acid sequence motif. The major activity of DEAH box proteins is to function as ATP-dependent RNA helicases. RNA helicases play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DHX8 is the human ortholog of the yeast Prp22 protein. DHX8 has been demonstrated to function in the release of RNA from the splicesome and the nuclear export of spliced mRNA. Alternate names for DHX8 include ATP-dependent RNA helicase DHX8, DEAH box protein 8, RNA helicase HRH1, DDX8, HRH1, PRP22, and PRPF22.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DHX8
Entrez
Uniprot