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DHX38 Antibody

Images

 
Western Blot: DHX38 Antibody [NBP1-85269] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: DHX38 Antibody [NBP1-85269] - Staining of human cell line U-2 OS shows positivity in nucleus.
Immunohistochemistry-Paraffin: DHX38 Antibody [NBP1-85269] - Staining of human esophagus shows strong cytoplasmic and nuclear positivity in squamous epithelial cells.
Western Blot: DHX38 Antibody [NBP1-85269] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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DHX38 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MDEGYDEFHNPLAYSSEDYVRRREQHLHKQKQKRISAQRRQINEDNERWETNRMLTSGVVHRLEVDEDFEEDNAAKVHLMVHNLVPPFLDGRIVFTKQPE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DHX38
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DHX38 Protein (NBP1-85269PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DHX38 Antibody

  • ATP-dependent RNA helicase DHX38
  • DDX38pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 38
  • DEAH (Asp-Glu-Ala-His) box polypeptide 38
  • DEAH box protein 38
  • EC 3.6.1
  • EC 3.6.4.13
  • KIAA0224pre-mRNA splicing factor ATP-dependent RNA helicase PRP16
  • PRP16 homolog of S.cerevisiae
  • PRP16hPrp16
  • PRPF16

Background

DHX38, also known as Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16, is a 140.5 kDa 1,227 amino acid protein that is utilized in multiple cellular processes, including ribosome and spliceosome assembly, as a member of the DEAD box protein family. Researchers are currently studying ties between the protein and North American Indian Childhood cirrhosis. The protein interacts in mRNA processing, mRNA splicing, RNA Polymerase II transcription, and spliceosome pathways with other proteins such as RBM10, SNRNP200, GPKOW, TXNL4A, and DHX16.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol DHX38