| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | 609809 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human DFF40/CAD Met1-Gln338 Accession # O76075 |
| Specificity | Detects human DFF40/CAD in direct ELISAs and Western blots. |
| Source | N/A |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DFFB |
| Purity Statement | Protein A or G purified from hybridoma culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.5 mg/mL in sterile PBS. |
DNA fragmentatation factor 40 kDa subunit (DFF40), also known as CAD, is a member of the CIDE domain-containing proteins. It is an apoptotic nuclease that is maintained in an inactive state by association with DFF45. Apoptotic signaling induces DFF45 cleavage and the release of DFF40. Active DFF40 exists as an oligomer and generates double stranded breaks in dsDNA. Alternate splicing of human DFF40 generates isoforms with substitutions and truncations either directly following the CIDE domain (which is aa 4‑80) or at the C-terminus. Full length human DFF40 (aa 1‑338) shares 76% and 74% aa sequence identity with mouse and rat DFF40, respectively.
![DFF40/CAD Antibody (609809) [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "DFF40/CAD Antibody (609809) [Unconjugated]")
| Publication using MAB6289 | Applications | Species |
|---|---|---|
| M Kijima, R Mizuta Histone H1 quantity determines the efficiencies of apoptotic DNA fragmentation and chromatin condensation Biomed. Res., 2019-01-01;40(1):51-56. 2019-01-01 [PMID: 30787263] (Western Blot, Human) | Western Blot | Human |
Secondary Antibodies |
Isotype Controls |
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