DEF8 Antibody (OTI4B6) - Azide and BSA Free Summary
| Immunogen |
Full length human recombinant protein of human DEF8 (NP_997397) produced in HEK293T cell. |
| Isotype |
IgG2a |
| Clonality |
Monoclonal |
| Host |
Mouse |
| Gene |
DEF8 |
| Purity |
Immunogen affinity purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- CyTOF-ready
- Flow Cytometry 1:100
- Immunocytochemistry/ Immunofluorescence 1:100
- Immunohistochemistry 1:150
- Immunohistochemistry-Paraffin
- Western Blot 1:500
|
| Theoretical MW |
58.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles. |
| Buffer |
Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative |
No Preservative |
| Concentration |
LYOPH |
| Purity |
Immunogen affinity purified |
| Reconstitution Instructions |
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Alternate Names for DEF8 Antibody (OTI4B6) - Azide and BSA Free
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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Secondary Antibodies
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Blogs on DEF8
![Western Blot: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DEF8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DEF8.](http://images.novusbio.com/fullsize/DEF8-Antibody-OTI4B6-Azide-and-BSA-Free-Western-Blot-NBP2-71969-img0001.jpg "Western Blot: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DEF8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DEF8.")
![Immunocytochemistry/Immunofluorescence: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DEF8.](http://images.novusbio.com/fullsize/DEF8-Antibody-OTI4B6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71969-img0004.jpg "Immunocytochemistry/Immunofluorescence: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DEF8.")
![Immunohistochemistry: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Staining of paraffin-embedded Human lymph node tissue using anti-DEF8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DEF8-Antibody-OTI4B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71969-img0008.jpg "Immunohistochemistry: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Staining of paraffin-embedded Human lymph node tissue using anti-DEF8 mouse monoclonal antibody.")
![Flow Cytometry: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Analysis of Hela cells, using anti-DEF8 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/DEF8-Antibody-OTI4B6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71969-img0002.jpg "Flow Cytometry: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Analysis of Hela cells, using anti-DEF8 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Immunohistochemistry: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DEF8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DEF8-Antibody-OTI4B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71969-img0005.jpg "Immunohistochemistry: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DEF8 mouse monoclonal antibody.")
![Immunohistochemistry: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DEF8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DEF8-Antibody-OTI4B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71969-img0006.jpg "Immunohistochemistry: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DEF8 mouse monoclonal antibody.")
![Immunohistochemistry: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Staining of paraffin-embedded Human liver tissue using anti-DEF8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DEF8-Antibody-OTI4B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71969-img0007.jpg "Immunohistochemistry: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Staining of paraffin-embedded Human liver tissue using anti-DEF8 mouse monoclonal antibody.")
![Immunohistochemistry: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Staining of paraffin-embedded Human lymphoma tissue using anti-DEF8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DEF8-Antibody-OTI4B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71969-img0009.jpg "Immunohistochemistry: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Staining of paraffin-embedded Human lymphoma tissue using anti-DEF8 mouse monoclonal antibody.")
![CyTOF-ready: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Analysis of Jurkat cells, using anti-DEF8 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/DEF8-Antibody-OTI4B6-Azide-and-BSA-Free-CyTOF-ready-NBP2-71969-img0003.jpg "CyTOF-ready: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Analysis of Jurkat cells, using anti-DEF8 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
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