![Western Blot: DEF8 Antibody (4B6) [NBP2-00574] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DEF8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DEF8.](http://images.novusbio.com/fullsize/DEF8-Antibody-4B6-Western-Blot-NBP2-00574-img0003.jpg "Western Blot: DEF8 Antibody (4B6) [NBP2-00574] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DEF8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DEF8.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI4B6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.58 mg/ml |
| Immunogen | Full length human recombinant protein of human DEF8 (NP_997397) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DEF8 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 58.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.58 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for DEF8 Antibody (NBP2-00574)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: DEF8 Antibody (4B6) [NBP2-00574] Staining of COS7 cells transiently transfected by pCMV6-ENTRY DEF8.](http://images.novusbio.com/fullsize/DEF8-Antibody-4B6-Immunocytochemistry-Immunofluorescence-NBP2-00574-img0009.jpg "Immunocytochemistry/Immunofluorescence: DEF8 Antibody (4B6) [NBP2-00574] Staining of COS7 cells transiently transfected by pCMV6-ENTRY DEF8.")
![Immunohistochemistry-Paraffin: DEF8 Antibody (4B6) [NBP2-00574] - Staining of paraffin-embedded Human lymphoma tissue using anti-DEF8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DEF8-Antibody-4B6-Immunohistochemistry-Paraffin-NBP2-00574-img0008.jpg "Immunohistochemistry-Paraffin: DEF8 Antibody (4B6) [NBP2-00574] - Staining of paraffin-embedded Human lymphoma tissue using anti-DEF8 mouse monoclonal antibody.")
![Flow Cytometry: DEF8 Antibody (4B6) [NBP2-00574] - Analysis of Jurkat cells, using anti-DEF8 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/DEF8-Antibody-4B6-Flow-Cytometry-NBP2-00574-img0002.jpg "Flow Cytometry: DEF8 Antibody (4B6) [NBP2-00574] - Analysis of Jurkat cells, using anti-DEF8 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Immunohistochemistry-Paraffin: DEF8 Antibody (4B6) [NBP2-00574] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DEF8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DEF8-Antibody-4B6-Immunohistochemistry-Paraffin-NBP2-00574-img0004.jpg "Immunohistochemistry-Paraffin: DEF8 Antibody (4B6) [NBP2-00574] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DEF8 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: DEF8 Antibody (4B6) [NBP2-00574] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DEF8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DEF8-Antibody-4B6-Immunohistochemistry-Paraffin-NBP2-00574-img0005.jpg "Immunohistochemistry-Paraffin: DEF8 Antibody (4B6) [NBP2-00574] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DEF8 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: DEF8 Antibody (4B6) [NBP2-00574] - Staining of paraffin-embedded Human liver tissue using anti-DEF8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DEF8-Antibody-4B6-Immunohistochemistry-Paraffin-NBP2-00574-img0006.jpg "Immunohistochemistry-Paraffin: DEF8 Antibody (4B6) [NBP2-00574] - Staining of paraffin-embedded Human liver tissue using anti-DEF8 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: DEF8 Antibody (4B6) [NBP2-00574] - Staining of paraffin-embedded Human lymph node tissue using anti-DEF8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DEF8-Antibody-4B6-Immunohistochemistry-Paraffin-NBP2-00574-img0007.jpg "Immunohistochemistry-Paraffin: DEF8 Antibody (4B6) [NBP2-00574] - Staining of paraffin-embedded Human lymph node tissue using anti-DEF8 mouse monoclonal antibody.")
![Flow Cytometry: DEF8 Antibody (4B6) [NBP2-00574] - Analysis of Hela cells, using anti-DEF8 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/DEF8-Antibody-4B6-Flow-Cytometry-NBP2-00574-img0001.jpg "Flow Cytometry: DEF8 Antibody (4B6) [NBP2-00574] - Analysis of Hela cells, using anti-DEF8 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
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