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DDX46 Antibody

Images

 
Independent Antibodies: Western Blot: DDX46 Antibody [NBP1-83565] - Analysis using Anti-DDX46 antibody NBP1-83565 (A) shows similar pattern to independent antibody NBP2-56227 (B).
Immunohistochemistry-Paraffin: DDX46 Antibody [NBP1-83565] - Staining of human testis shows strong nuclear positivity in seminiferous tubules.
Western Blot: DDX46 Antibody [NBP1-83565] - Analysis in human cell line HEK 293.
Genetic Strategies: Western Blot: DDX46 Antibody [NBP1-83565] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-DDX46 antibody. Remaining relative ...read more
Western Blot: DDX46 Antibody [NBP1-83565] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DDX46 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KEGNEMEGEELDPLDAYMEEVKEEVKKFNMRSVKGGGGNEKKSGPTVTKVVTVVTTKKAVVDSDKKKGELMENDQDAM
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX46
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
DDX46 Protein (NBP1-83565PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DDX46 Antibody

  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 46
  • DEAD box protein 46
  • EC 3.6.1
  • EC 3.6.4.13
  • FLJ25329
  • KIAA0801probable ATP-dependent RNA helicase DDX46
  • MGC9936
  • PRP5 homolog
  • Prp5
  • Prp5-like DEAD-box protein
  • PRPF5
  • RNA helicase

Background

DDX46 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the 17S U2 snRNP complex; it plays an important role in pre-mRNA splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DDX46