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DDOST Antibody

Images

 
Western Blot: DDOST Antibody [NBP2-38560] - Analysis in human cell line CAPAN-2.
Immunocytochemistry/ Immunofluorescence: DDOST Antibody [NBP2-38560] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
Immunohistochemistry-Paraffin: DDOST Antibody [NBP2-38560] - Staining of human duodenum shows cytoplasmic positivity in leukocytes and glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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DDOST Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: NYELAVALSRWVFKEEGVLRVGPVSHHRVGETAPPNAYTVTDLVEYSIVIQQLSNGKWVPFDGDDIQLEFVRIDPFVRTFLKKKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHTQY
Predicted Species
Mouse (97%), Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDOST
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DDOST Protein (NBP2-38560PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DDOST Antibody

  • advanced glycation endproduct receptor 1
  • DDOST 48 kDa subunit
  • dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
  • dolichyl-diphosphooligosaccharide-protein glycosyltransferase
  • dolichyl-diphosphooligosaccharide--protein glycosyltransferase
  • dolichyl-diphosphooligosaccharide-protein glycotransferase
  • EC 2.4.1.119
  • KIAA0115MGC2191
  • Oligosaccharyl transferase 48 kDa subunit
  • oligosaccharyltransferase 48 kDa subunit
  • OST48AGER1
  • OSTOKSWcl45
  • WBP1

Background

DDOST encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol DDOST
Uniprot