![Western Blot: DDO Antibody [NBP1-85884] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-150](http://images.novusbio.com/fullsize/DDO-Antibody-Western-Blot-NBP1-85884-img0005.jpg "Western Blot: DDO Antibody [NBP1-85884] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-150")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: KGDWNLSPDAENSREILSRCCALEPSLHGACNIREKVGLRPYRPGVRLQTELLARDGQRLPVVHHYGHGSGGISVHWGTALEAARLVSECVHALR |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DDO |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
![Neutralization Prolactin Antibody [Unconjugated]](https://images.novusbio.com/images2/Prolactin_AF1445_Block_Neutralize_8779.jpg)
![Western Blot Prolactin Antibody [Unconjugated]](https://images.novusbio.com/images2/Prolactin_AF1445_Western_Blot_20179.jpg)
![Immunohistochemistry Prolactin Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af1445_mouse-rat-prolactin-affinity-purified-polyclonal-ab-immunohistochemistry-19202393922..jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DDO |
![Immunocytochemistry/Immunofluorescence: DDO Antibody [NBP1-85884] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/DDO-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85884-img0008.jpg "Immunocytochemistry/Immunofluorescence: DDO Antibody [NBP1-85884] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.")
![Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/DDO-Antibody-Immunohistochemistry-Paraffin-NBP1-85884-img0012.jpg "Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884] - Staining of human cerebral cortex.")
![Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884] - Staining of human appendix shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DDO-Antibody-Immunohistochemistry-Paraffin-NBP1-85884-img0007.jpg "Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884] - Staining of human appendix shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884] - Staining of human cerebral cortex, colon, lymph node and urinary bladder using Anti-DDO antibody NBP1-85884 (A) shows similar protein distribution across tissues to independent antibody NBP2-32684 (B).](http://images.novusbio.com/fullsize/DDO-Antibody-Immunohistochemistry-Paraffin-NBP1-85884-img0009.jpg "Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884] - Staining of human cerebral cortex, colon, lymph node and urinary bladder using Anti-DDO antibody NBP1-85884 (A) shows similar protein distribution across tissues to independent antibody NBP2-32684 (B).")
![Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884] - Staining of human colon.](http://images.novusbio.com/fullsize/DDO-Antibody-Immunohistochemistry-Paraffin-NBP1-85884-img0010.jpg "Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884] - Staining of human colon.")
![Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884] - Staining of human lymph node.](http://images.novusbio.com/fullsize/DDO-Antibody-Immunohistochemistry-Paraffin-NBP1-85884-img0011.jpg "Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884] - Staining of human lymph node.")
![Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884] - Staining of human urinary bladder.](http://images.novusbio.com/fullsize/DDO-Antibody-Immunohistochemistry-Paraffin-NBP1-85884-img0013.jpg "Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884] - Staining of human urinary bladder.")
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![Western Blot BRCA2 Antibody (234403) [Unconjugated]](https://images.novusbio.com/images2/BRCA2_MAB2476_Western_Blot_6033.jpg)
![Immunohistochemistry BRCA2 Antibody (234403) [Unconjugated]](https://images.novusbio.com/images2/mab2476_human-brca2-mab-clone-234403-immunohistochemistry-10520211657.jpg)
![Western Blot BRCA2 Antibody (234403) [Unconjugated]](https://images.novusbio.com/images/mab2476_human-brca2-mab-clone-234403-2282024172111.jpg)
![N/A Haptoglobin [HRP]](https://images.novusbio.com/images2/Haptoglobin_DHAPG0_ELISA_461.jpg)
![N/A Haptoglobin [HRP]](https://images.novusbio.com/images2/DATA_Haptoglobin_DHAPG0_ELISA_709.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")