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DBT Antibody (OTI1G2)

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Western Blot: DBT Antibody (OTI1G2) [NBP2-02042] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DBT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DBT.
Western Blot: DBT Antibody (OTI1G2) [NBP2-02042] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DBT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: DBT Antibody (OTI1G2) [NBP2-02042] - Anti-DBT mouse monoclonal antibody (NBP2-02042) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DBT.
Flow Cytometry: DBT Antibody (OTI1G2) [NBP2-02042] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DBT antibody, and then analyzed ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
OTI1G2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70553AF350
Alexa Fluor 405
NBP2-70553AF405
Alexa Fluor 488
NBP2-70553AF488
Alexa Fluor 532
NBP2-70553AF532
Alexa Fluor 594
NBP2-70553AF594
Alexa Fluor 647
NBP2-70553AF647
Alexa Fluor 700
NBP2-70553AF700
Alexa Fluor 750
NBP2-70553AF750
Allophycocyanin
NBP2-70553APC
Biotin
NBP2-70553B
CoraFluor 1
NBP2-70553CL1
DyLight 350
NBP2-70553UV
DyLight 405
NBP2-70553V
DyLight 488
NBP2-70553G
DyLight 550
NBP2-70553R
DyLight 594
NBP2-70553DL594
DyLight 650
NBP2-70553C
DyLight 680
NBP2-70553FR
DyLight 755
NBP2-70553IR
FITC
NBP2-70553F
HRP
NBP2-70553H
Janelia Fluor 525
NBP2-70553JF525
Janelia Fluor 549
NBP2-70553JF549
Janelia Fluor 646
NBP2-70553JF646
Janelia Fluor 669
NBP2-70553JF669
PE
NBP2-70553PE
PerCP
NBP2-70553PCP
mFluor Violet 450 SE
NBP2-70553MFV450
mFluor Violet 500 SE
NBP2-70553MFV500
mFluor Violet 610 SE
NBP2-70553MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-70553
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DBT Antibody (OTI1G2) Summary

Immunogen
Full length human recombinant protein of human DBT(NP_001909) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DBT
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:2000
Theoretical MW
46.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DBT Antibody (OTI1G2)

  • BCATE2BCKAD E2 subunit
  • BCKADE2
  • BCKAD-E2
  • branched chain acyltransferase, E2 component
  • Branched-chain alpha-keto acid dehydrogenase complex component E2
  • Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto aciddehydrogenase complex
  • dihydrolipoamide branched chain transacylase (E2 component of branched chainketo acid dehydrogenase complex; maple syrup urine disease)
  • dihydrolipoamide branched chain transacylase E2
  • Dihydrolipoamide branched chain transacylase
  • dihydrolipoyl transacylase
  • Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase
  • E2 component of branched chain alpha-keto acid dehydrogenase complex
  • E2
  • E2B
  • EC 2.3.1.168
  • lipoamide acyltransferase component of branched-chain alpha-keto aciddehydrogenase complex, mitochondrial
  • lipoamide acyltransferase component of mitochondrial branched-chain alpha-ketoacid dehydrogenase complex
  • MGC9061
  • mitochondrial branched chain alpha-keto acid dehydrogenase transacylase subunit(E2b)

Background

The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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DBT Antibody
(OTI1G2)
NBP2-02042
DBT Antibody (OTI1G2)
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF

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Bioinformatics

Gene Symbol DBT
Entrez