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![Immunohistochemistry: DAP Kinase 1 Antibody (SR1225) [NBP3-21649] - Immunohistochemical analysis of paraffin-embedded human breast cancer, using NBP3-21649.](http://images.novusbio.com/fullsize/nbp3-21649_rabbit-dap-kinase-1-mab-sr1225-5720231595523.jpg "Immunohistochemistry: DAP Kinase 1 Antibody (SR1225) [NBP3-21649] - Immunohistochemical analysis of paraffin-embedded human breast cancer, using NBP3-21649.")
![Western Blot: DAP Kinase 1 Antibody (SR1225) [NBP3-21649] - Western blot analysis using NBP3-21649 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21649_rabbit-dap-kinase-1-mab-sr1225-57202316494246.jpg)
![Immunocytochemistry/Immunofluorescence: DAP Kinase 1 Antibody (SR1225) [NBP3-21649] - Immunofluorescent analysis of Hela cells, using NBP3-21649.](http://images.novusbio.com/fullsize/nbp3-21649_rabbit-dap-kinase-1-mab-sr1225-6720231051262.jpg)
![Western Blot: DAP Kinase 1 [p Ser308] Antibody (DKPS308) [NB120-10524] - Whole extract of HEK-293 cells overexpressing human DAP Kinase was separated on SDS-PAGE and probed with 1 ug/mL Monoclonal Anti-phospho-DAP-Kinase (pSer308) Clone: DKPS308. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.](http://images.novusbio.com/fullsize/DAP-Kinase-1-p-Ser308-Antibody-DKPS308-Western-Blot-NB120-10524-img0001.jpg "Western Blot: DAP Kinase 1 [p Ser308] Antibody (DKPS308) [NB120-10524] - Whole extract of HEK-293 cells overexpressing human DAP Kinase was separated on SDS-PAGE and probed with 1 ug/mL Monoclonal Anti-phospho-DAP-Kinase (pSer308) Clone: DKPS308. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.")
![Western Blot: DAP Kinase 1 Antibody [NBP3-38180] - Western blot analysis of various lysates using DAP Kinase 1(DAPK1) Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38180_rabbit-dap-kinase-1-pab-1012202411353089.jpg "Western Blot: DAP Kinase 1 Antibody [NBP3-38180] - Western blot analysis of various lysates using DAP Kinase 1(DAPK1) Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.")
![Western Blot: DAP Kinase 1 Antibody (12B2-R) [NBP3-32256] - Western blot analysis of DAP Kinase 1 on different lysates with Mouse anti-DAP Kinase 1 antibody (NBP3-32256) at 1/2,000 dilution. Lane 1: A549 cell lysate Lane 2: TF-1 cell lysate Lane 3: HepG2 cell lysate Lane 4: Human brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 160 kDa Observed band size: 160 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32256) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32256_mouse-dap-kinase-1-mab-12b2-r-278202415212414.jpg "Western Blot: DAP Kinase 1 Antibody (12B2-R) [NBP3-32256] - Western blot analysis of DAP Kinase 1 on different lysates with Mouse anti-DAP Kinase 1 antibody (NBP3-32256) at 1/2,000 dilution. Lane 1: A549 cell lysate Lane 2: TF-1 cell lysate Lane 3: HepG2 cell lysate Lane 4: Human brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 160 kDa Observed band size: 160 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32256) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DAP Kinase 1 Antibody (12B2-R) [NBP3-32256] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-DAP Kinase 1 antibody (NBP3-32256) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32256) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32256_mouse-dap-kinase-1-mab-12b2-r-29820241358550.jpg)
![Sandwich ELISA: DAP Kinase 1 Antibody (2E7) [H00001612-M01] - Detection limit for recombinant GST tagged DAPK1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DAP-Kinase-1-Antibody-2E7-Sandwich-ELISA-H00001612-M01-img0001.jpg "Sandwich ELISA: DAP Kinase 1 Antibody (2E7) [H00001612-M01] - Detection limit for recombinant GST tagged DAPK1 is approximately 0.1ng/ml as a capture antibody.")
![Proximity Ligation Assay: DAP Kinase 1 Antibody (2E7) [H00001612-M01] - Analysis of protein-protein interactions between MAPK3 and DAPK1. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-DAPK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/DAP-Kinase-1-Antibody-2E7-Proximity-Ligation-Assay-H00001612-M01-img0002.jpg)
![Western Blot: DAP Kinase 1 Antibody (3E10G3) [NBP3-15382] - Western blot analysis of extracts of various cell lines, using DAP Kinase 1 Rabbit mAb (NBP3-15382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/DAP-Kinase-1-Antibody-3E10G3-Western-Blot-NBP3-15382-img0001.jpg "Western Blot: DAP Kinase 1 Antibody (3E10G3) [NBP3-15382] - Western blot analysis of extracts of various cell lines, using DAP Kinase 1 Rabbit mAb (NBP3-15382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: DAP Kinase 1 Antibody [NBP2-38580] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/DAP-Kinase-1-Antibody-Immunohistochemistry-NBP2-38580-img0001.jpg "Immunohistochemistry-Paraffin: DAP Kinase 1 Antibody [NBP2-38580] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.")
![Immunohistochemistry-Paraffin: DAP Kinase 1 Antibody [NBP2-38468] - Staining of human vulva/anal skin shows distinct cytoplasmic positivity in basal layer of epidermis.](http://images.novusbio.com/fullsize/DAP-Kinase-1-Antibody-Immunohistochemistry-Paraffin-NBP2-38468-img0002.jpg "Immunohistochemistry-Paraffin: DAP Kinase 1 Antibody [NBP2-38468] - Staining of human vulva/anal skin shows distinct cytoplasmic positivity in basal layer of epidermis.")
![Western Blot: DAP Kinase 1 Antibody [NBP1-59001] - THP-1 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/DAP-Kinase-1-Antibody-Western-Blot-NBP1-59001-img0002.jpg "Western Blot: DAP Kinase 1 Antibody [NBP1-59001] - THP-1 cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: DAP Kinase 1 Overexpression Lysate (Adult Normal) [NBL1-09716] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DAP Kinase 1.](http://images.novusbio.com/fullsize/DAP-Kinase-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09716-img0002.jpg "Western Blot: DAP Kinase 1 Overexpression Lysate (Adult Normal) [NBL1-09716] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DAP Kinase 1.")
![ELISA: Human DAP Kinase 1 ELISA Kit (Colorimetric) [NBP3-27326] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-27326_human-dap-kinase-1-elisa-kit-colorimetric-11420241031212.png "ELISA: Human DAP Kinase 1 ELISA Kit (Colorimetric) [NBP3-27326] - Standard Curve Reference")