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Cytosolic beta-Glucosidase/GBA3 Antibody [Unconjugated]

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Western blot shows lysates of human kidney (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Cytosolic beta -Glucosidase/GBA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5969) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cytosolic beta -Glucosidase/GBA3 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human kidney (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Cytosolic beta -Glucosidase/GBA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5969) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5969-SP
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Cytosolic beta-Glucosidase/GBA3 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cytosolic beta ‑Glucosidase/GBA3
Accession # Q9H227
Specificity
Detects human Cytosolic beta ‑Glucosidase/GBA3 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
GBA3
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cytosolic beta-Glucosidase/GBA3 Antibody [Unconjugated]

  • CBG
  • CBGL1
  • CBGL1GLUC
  • Cytosolic betaGlucosidase
  • Cytosolic beta-Glucosidase
  • Cytosolic beta-glucosidase-like protein 1
  • EC 3.2.1.21
  • GBA3
  • GLUC
  • glucosidase, beta, acid 3 (cytosolic)
  • klotho-related protein
  • KLrP
  • MGC104276
  • MGC126878

Background

There are three beta‑glucosidases (GBA) in human genome. GBA1 endodes a lysosomal membrane protein that cleaves the beta‑glucosidic linkage of glucosylceramide (1). GBA2 encodes a microsomal beta-glucosidase that catalyzes the hydrolysis of bile acid 3-O-glucosides (2). GBA3 is a cytosolic beta‑glucosidase and is predominantly expressed in liver. GBA3 efficiently hydrolyzes beta‑D‑glucoside and beta‑D‑galactoside, but not any known physiological beta‑glycoside, suggesting that it may be involved in detoxification of plant glycosides (3). GBA3 also has significant neutral glycosylceramidase activity, suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (4). At the protein level, GBA3 shows significant homology (>40%) with Klotho protein that is known for its association with aging process (3, 4).

  1. Tybulewicz, V.L. et al. (1992) Nature 357:407.
  2. Matern, H. et al. (2001) J. Biol. Chem. 276:37929.
  3. de Graaf, M. et al. (2001) Biochem. J. 356:907.
  4. Hayashi, Y. et al. (2007) J. Biol. Chem. 282:30889.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GBA3
Uniprot