![Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/1576R) - Azide and BSA Free [NBP2-54589] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, HMW Antibody (KRTH/1576R).](http://images.novusbio.com/fullsize/Cytokeratin-HMW-Antibody-KRTH-1576R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54589-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/1576R) - Azide and BSA Free [NBP2-54589] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, HMW Antibody (KRTH/1576R).")
| Reactivity | Hu, CaSpecies Glossary |
| Applications | IHC |
| Clone | KRTH/1576R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of recombinant monoclonal antibody purified by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53164). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant full-length human Cytokeratin, HMW protein (Uniprot: Q01546) |
| Localization | Cytoplasmic |
| Marker | Epithelial Marker |
| Specificity | This monoclonal antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | KRT76 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | KRT76 |
![Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/1576R) - Azide and BSA Free [NBP2-54589] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, HMW Recombinant Rabbit Monoclonal Antibody (KRTH/1576R).](http://images.novusbio.com/fullsize/Cytokeratin-HMW-Antibody-KRTH-1576R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54589-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/1576R) - Azide and BSA Free [NBP2-54589] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, HMW Recombinant Rabbit Monoclonal Antibody (KRTH/1576R).")
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