![Western Blot: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Analysis of Cytokeratin 8 on different lysates with Rabbit anti-Cytokeratin 8 antibody at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Western-Blot-NBP2-67468-img0009.jpg "Western Blot: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Analysis of Cytokeratin 8 on different lysates with Rabbit anti-Cytokeratin 8 antibody at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | SU0338 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human Cytokeratin 8 aa 321-370 / 483. (SwissProt: P05787 Human; SwissProt: P11679 Mouse) |
| Localization | Nucleoplasm, Nucleus matrix, Cytoplasm. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | KRT8 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 8 Antibody (NBP2-67468)Find related products by research area.
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Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen... Read full blog post. |
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| Gene Symbol | KRT8 |
![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Analysis of SK-Br-3 cells labeling Cytokeratin 8 (and then blocked with 2% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody Cytokeratin 8 (red) at 1/50 dilution and Beta-tubulin (yellow) at 1/500 dilution at +4 overnight. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Immunocytochemistry-Immunofluorescence-NBP2-67468-img0010.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Analysis of SK-Br-3 cells labeling Cytokeratin 8 (and then blocked with 2% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody Cytokeratin 8 (red) at 1/50 dilution and Beta-tubulin (yellow) at 1/500 dilution at +4 overnight. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 8 (washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (red) at 1/50 dilution and Vimentin (green) at 1/500 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Immunohistochemistry-Paraffin-NBP2-67468-img0012.jpg "Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 8 (washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (red) at 1/50 dilution and Vimentin (green) at 1/500 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Flow Cytometry: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Flow cytometric analysis of Cytokeratin 8 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Flow-Cytometry-NBP2-67468-img0008.jpg "Flow Cytometry: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Flow cytometric analysis of Cytokeratin 8 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).")
![Western Blot: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Cytokeratin 18 Antibody (SU0338) [NBP2-67468] - Analysis of Cytokeratin 8 on different lysates using anti-Cytokeratin 8 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: A431](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Western-Blot-NBP2-67468-img0007.jpg "Western Blot: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Cytokeratin 18 Antibody (SU0338) [NBP2-67468] - Analysis of Cytokeratin 8 on different lysates using anti-Cytokeratin 8 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: A431")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Staining Cytokeratin 8 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Immunocytochemistry-Immunofluorescence-NBP2-67468-img0002.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Staining Cytokeratin 8 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Staining Cytokeratin 8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Immunocytochemistry-Immunofluorescence-NBP2-67468-img0003.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Staining Cytokeratin 8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Staining Cytokeratin 8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Immunocytochemistry-Immunofluorescence-NBP2-67468-img0004.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Staining Cytokeratin 8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Immunohistochemistry-Paraffin-NBP2-67468-img0005.jpg "Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Immunohistochemistry-Paraffin-NBP2-67468-img0006.jpg "Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 8 (washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (red) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Immunohistochemistry-Paraffin-NBP2-67468-img0011.jpg "Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 8 (washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (red) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Flow Cytometry: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Analysis of Hela cells with Cytokeratin 8 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Flow-Cytometry-NBP2-67468-img0001.jpg "Flow Cytometry: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Analysis of Hela cells with Cytokeratin 8 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")