![Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - A549 cell segregation and proliferation inside the spheroids. Four- day-old heterospheroids (as indicated) were double-stained with anti-human cytokeratin 7 (stained A549 cells, red) and anti-mouse beta1 integrin (stained MEFs, green) antibodies. Size bars = 100 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0103173) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-R17-S-Immunocytochemistry-Immunofluorescence-NBP1-30152-img0009.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - A549 cell segregation and proliferation inside the spheroids. Four- day-old heterospheroids (as indicated) were double-stained with anti-human cytokeratin 7 (stained A549 cells, red) and anti-mouse beta1 integrin (stained MEFs, green) antibodies. Size bars = 100 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0103173) licensed under a CC-BY license.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clone | R17-S |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody is immunoaffinity purified with immunogenic peptide as a ligand. |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Peptide derived from N-terminal sequence of human cytokeratin 7. Antibody recognizes the epitope between Ala22 - Ser38. |
| Epitope | Ala22 - Ser38 |
| Specificity | This antibody is specific for the N terminus o Cytokeratin 7 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | KRT7 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 25076207). |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | 20mM Tris-HCl (pH 8.0) and 20mg/ml BSA |
| Preservative | 0.05% Sodium Azide |
| Purity | Immunogen affinity purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - Spheroid migration on collagen I monolayers and invasion in 3D collagen gels. Fluorescence immunostaining of the spheroids on coverslips after 24 h and migration distance of A549 from the heterospheroids. Spheroids were double stained with antibodies towards human Cytokeratin 7 (stained A549 cells, red) and mouse beta1 integrin (stained MEFs, green). Six of each type of spheroids were stained. The maximum migration distance (d) of A549 cells were measured on the photographs as indicated in the figure and calculated according to size bar. Size bars = 100 um. **** p<0.0001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0103173) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-R17-S-Immunocytochemistry-Immunofluorescence-NBP1-30152-img0011.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - Spheroid migration on collagen I monolayers and invasion in 3D collagen gels. Fluorescence immunostaining of the spheroids on coverslips after 24 h and migration distance of A549 from the heterospheroids. Spheroids were double stained with antibodies towards human Cytokeratin 7 (stained A549 cells, red) and mouse beta1 integrin (stained MEFs, green). Six of each type of spheroids were stained. The maximum migration distance (d) of A549 cells were measured on the photographs as indicated in the figure and calculated according to size bar. Size bars = 100 um. **** p<0.0001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0103173) licensed under a CC-BY license.")
![Immunohistochemistry: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - Analysis of immunofluorecence stained collagen type I fibrils in primary tumors. The amount of stroma versus tumor cells demonstrates significantly less cancer cells in the HBOT group compared to control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0183254), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-R17-S-Immunohistochemistry-NBP1-30152-img0006.jpg "Immunohistochemistry: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - Analysis of immunofluorecence stained collagen type I fibrils in primary tumors. The amount of stroma versus tumor cells demonstrates significantly less cancer cells in the HBOT group compared to control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0183254), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - Pulmonary adenocarcinoma (A), ductal pancreatic adenocarcinoma (B) and ductal carcinoma of the breast (C) showing diffuse CK7 positivity. Formalin fixed, paraffin embedded human tissues (4 um sections) stained with anti - Cytokeratin 7 monospecific clonal antibody.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-R17-S-Immunohistochemistry-Paraffin-NBP1-30152-img0005.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - Pulmonary adenocarcinoma (A), ductal pancreatic adenocarcinoma (B) and ductal carcinoma of the breast (C) showing diffuse CK7 positivity. Formalin fixed, paraffin embedded human tissues (4 um sections) stained with anti - Cytokeratin 7 monospecific clonal antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")