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Cytokeratin 6 Antibody (KRT6/8198R) - Azide and BSA Free

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Formalin-fixed, paraffin-embedded human lung stained with Cytokeratin 6 antibody (KRT6/8198R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human lung stained with Cytokeratin 6 antibody (KRT6/8198R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human prostate stained with Cytokeratin 6 antibody (KRT6/8198R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
KRT6/8198R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-20716AF350
Alexa Fluor 405
NBP3-20716AF405
Alexa Fluor 488
NBP3-20716AF488
Alexa Fluor 532
NBP3-20716AF532
Alexa Fluor 594
NBP3-20716AF594
Alexa Fluor 647
NBP3-20716AF647
Alexa Fluor 700
NBP3-20716AF700
Alexa Fluor 750
NBP3-20716AF750
Allophycocyanin
NBP3-20716APC
Biotin
NBP3-20716B
CoraFluor 1
NBP3-20716CL1
DyLight 350
NBP3-20716UV
DyLight 405
NBP3-20716V
DyLight 488
NBP3-20716G
DyLight 550
NBP3-20716R
DyLight 594
NBP3-20716DL594
DyLight 650
NBP3-20716C
DyLight 680
NBP3-20716FR
DyLight 755
NBP3-20716IR
FITC
NBP3-20716F
HRP
NBP3-20716H
Janelia Fluor 525
NBP3-20716JF525
Janelia Fluor 549
NBP3-20716JF549
Janelia Fluor 585
NBP3-20716JF585
Janelia Fluor 635
NBP3-20716JF635
Janelia Fluor 646
NBP3-20716JF646
Janelia Fluor 669
NBP3-20716JF669
PE
NBP3-20716PE
PerCP
NBP3-20716PCP
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Cytokeratin 6 Antibody (KRT6/8198R) - Azide and BSA Free Summary

Description
Positive Controls: Human tonsil or basal cell carcinoma (BCC).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fragment (around aa1-200) of human Cytokeratin 6 (exact sequence is proprietary)
Localization
Cytoplasm
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
KRT6A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Buffer
10mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Cytokeratin 6 Antibody (KRT6/8198R) - Azide and BSA Free

  • Allergen Hom s 5
  • CK6A
  • CK-6A
  • CK6C
  • CK6D
  • CK-6D
  • cytokeratin 6A
  • cytokeratin 6C
  • cytokeratin 6D
  • Cytokeratin-6A
  • Cytokeratin-6D
  • K6A
  • K6C
  • K6D
  • keratin 6A
  • keratin 6C
  • keratin 6D
  • keratin, epidermal type II, K6A
  • keratin, type II cytoskeletal 6A
  • Keratin-6A
  • KRT6C
  • KRT6D
  • Type-II keratin Kb6

Background

Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation related keratins). Keratin 6 is found in hair follicles, neck squamous cell carcinomas, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyperproliferative situations. Epidermal injury results in activation of keratinocytes which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation. There are at least six isoforms of human type II keratin 6 (K6), K6A being the most abundant representing about 77% of all forms found in epithelia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol KRT6A