![Western Blot: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Western-Blot-NBP2-44948-img0013.jpg "Western Blot: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (KRT18/834).")
| Reactivity | Hu, Bv(-), Ca(-), Ha(-), Mu(-), Po(-), Rt(-), Sh(-)Species Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | KRT18/834 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47985) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant full-length human Cytokeratin 18 protein (Uniprot: P05783) |
| Localization | Cytoplasmic |
| Marker | Epithelial Marker |
| Specificity | This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT18 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
![Simple Western alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](https://images.novusbio.com/images2/16253.jpg)
![Knockout Validated alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](https://images.novusbio.com/images2/alphaFetoprotein_MAB1368_Knockout_Validated_21503.jpg)
![Knockout Validated alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](https://images.novusbio.com/images2/alphaFetoprotein_MAB1368_Knockout_Validated_22402.jpg)
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 18 Antibody (NBP2-44948)Find related products by research area.
|
|
Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Immunofluorescent staining of MCF cells using Cytokeratin 18 Antibody (KRT18/834). Green (CF488) and Red Dot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Immunocytochemistry-Immunofluorescence-NBP2-44948-img0010.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Immunofluorescent staining of MCF cells using Cytokeratin 18 Antibody (KRT18/834). Green (CF488) and Red Dot is used to label the nuclei Red.")
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with Cytokeratin 18 Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Immunohistochemistry-Paraffin-NBP2-44948-img0011.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with Cytokeratin 18 Antibody (KRT18/834).")
![Flow Cytometry: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells using Cytokeratin 18 Antibody (KRT18/834) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Flow-Cytometry-NBP2-44948-img0009.jpg "Flow Cytometry: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells using Cytokeratin 18 Antibody (KRT18/834) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)")
![Western Blot: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Western Blot Analysis of A) human stomach B) human intestine using Cytokeratin 18 Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Western-Blot-NBP2-44948-img0012.jpg "Western Blot: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Western Blot Analysis of A) human stomach B) human intestine using Cytokeratin 18 Antibody (KRT18/834).")
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Human Breast Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Immunohistochemistry-Paraffin-NBP2-44948-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Human Breast Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).")
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Human Colon Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Immunohistochemistry-Paraffin-NBP2-44948-img0003.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Human Colon Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).")
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Human Lung Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Immunohistochemistry-Paraffin-NBP2-44948-img0004.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Human Lung Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).")
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Human Prostate Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Immunohistochemistry-Paraffin-NBP2-44948-img0005.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Human Prostate Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).")
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Human Skin stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Immunohistochemistry-Paraffin-NBP2-44948-img0006.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Human Skin stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).")
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Human Thyroid Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Immunohistochemistry-Paraffin-NBP2-44948-img0007.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Human Thyroid Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).")
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![Immunohistochemistry Desmin Antibody [Unconjugated]](https://images.novusbio.com/images2/Desmin_AF3844_Immunohistochemistry_10689.jpg)
![Immunohistochemistry Desmin Antibody [Unconjugated]](https://images.novusbio.com/images2/Desmin_AF3844_Immunohistochemistry_6808.jpg)
![Immunocytochemistry/ Immunofluorescence Desmin Antibody [Unconjugated]](https://images.novusbio.com/images/af3844_human-mouse-desmin-affinity-purified-polyclonal-ab-41202410331920.jpg)
![Bioactivity HGF [Unconjugated]](https://images.novusbio.com/images2/HGF_294HG_3253.jpg)
![Cell Culture HGF [Unconjugated]](https://images.novusbio.com/images/294-hg_recombinant-human-hgf-protein-cell-culture-252023111456.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/DATA_Kallikrein_3_DKK300_ELISA_729.jpg)
![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/DATA_Kallikrein_3_DKK300_ELISA_730.jpg)
![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/Kallikrein_3_DKK300_ELISA_124.jpg)
![Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_6973.jpg)
![Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_9671.jpg)
![Immunoprecipitation CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412513815.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")