![Immunohistochemistry-Paraffin: Cytokeratin 10/13 Antibody (DE-K13) - IHC-Prediluted [NBP2-48185] - Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin 10/13 Monoclonal Antibody (DE-K13)](http://images.novusbio.com/fullsize/Cytokeratin-10-13-Antibody-DE-K13-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48185-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 10/13 Antibody (DE-K13) - IHC-Prediluted [NBP2-48185] - Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin 10/13 Monoclonal Antibody (DE-K13)")
| Reactivity | Hu, FeSpecies Glossary |
| Applications | IHC |
| Clone | DE-K13 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | IHC-Prediluted |
| Description | The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining. |
| Immunogen | Cytoskeletal preparation extracted from human ectocervical epithelium |
| Localization | Cytoplasmic |
| Specificity | This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this monoclonal antibody serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT10 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Hu-chromosome location: 17q21.2 Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | KRT10 |
