![Immunocytochemistry/Immunofluorescence: Cytokeratin 10/13 Antibody (DE-K13) [NBP2-45256] - Immunofluorescent analysis of MeOH-fixed MCF-7 cells using Cytokeratin 10/13Mouse Monoclonal Antibody (DE-K13) followed by goat anti-mouse IgG-CF488. Nuclei counterstained with RedDot.](http://images.novusbio.com/fullsize/Cytokeratin-10-13-Antibody-DE-K13-Immunocytochemistry-Immunofluorescence-NBP2-45256-img0002.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 10/13 Antibody (DE-K13) [NBP2-45256] - Immunofluorescent analysis of MeOH-fixed MCF-7 cells using Cytokeratin 10/13Mouse Monoclonal Antibody (DE-K13) followed by goat anti-mouse IgG-CF488. Nuclei counterstained with RedDot.")
| Reactivity | Hu, FeSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, ICC/IF |
| Clone | DE-K13 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11497) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Cytoskeletal preparation extracted from human ectocervical epithelium |
| Localization | Cytoplasmic |
| Specificity | This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this monoclonal antibody serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT10 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Cytokeratin 10/13 Antibody (DE-K13) [NBP2-45256] - Human Tonsil stained with Cytokeratin 10/13 Monoclonal Antibody (DE-K13)](http://images.novusbio.com/fullsize/Cytokeratin-10-13-Antibody-DE-K13-Immunohistochemistry-Paraffin-NBP2-45256-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 10/13 Antibody (DE-K13) [NBP2-45256] - Human Tonsil stained with Cytokeratin 10/13 Monoclonal Antibody (DE-K13)")
