![Western Blot: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Analysis of Cytokeratin 1/10 on different lysates with Rabbit anti-Cytokeratin 1/10 antibody at 1/500 dilution. Lane 1: Rat skin tissue lysate Lane 2: Human skin tissue lysate. Lysates/proteins at 20 ug/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa. Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Cytokeratin-1-10-Antibody-ST05-43-Western-Blot-NBP2-67544-img0006.jpg "Western Blot: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Analysis of Cytokeratin 1/10 on different lysates with Rabbit anti-Cytokeratin 1/10 antibody at 1/500 dilution. Lane 1: Rat skin tissue lysate Lane 2: Human skin tissue lysate. Lysates/proteins at 20 ug/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa. Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | ST05-43 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human Cytokeratin 1/10 aa 541-584 / 584. (SwissProt: P13645 Human; SwissProt: P02535 Mouse; SwissProt: Q6IFW6 Rat) |
| Localization | Cytoplasm. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | KRT10 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 1/10 Antibody (NBP2-67544)Find related products by research area.
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| Gene Symbol | KRT10 |
![Immunocytochemistry/Immunofluorescence: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Staining Cytokeratin 10 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-1-10-Antibody-ST05-43-Immunocytochemistry-Immunofluorescence-NBP2-67544-img0001.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Staining Cytokeratin 10 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-Cytokeratin 1/10 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-1-10-Antibody-ST05-43-Immunohistochemistry-Paraffin-NBP2-67544-img0010.jpg "Immunohistochemistry-Paraffin: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-Cytokeratin 1/10 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Analysis of Cytokeratin 10 on Hela cell lysates using anti-Cytokeratin 10 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Cytokeratin-1-10-Antibody-ST05-43-Western-Blot-NBP2-67544-img0004.jpg "Western Blot: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Analysis of Cytokeratin 10 on Hela cell lysates using anti-Cytokeratin 10 antibody at 1/1,000 dilution.")
![Western Blot: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Western blot analysis of Cytokeratin 1/10 on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 h](http://images.novusbio.com/fullsize/Cytokeratin-1-10-Antibody-ST05-43-Western-Blot-NBP2-67544-img0005.jpg "Western Blot: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Western blot analysis of Cytokeratin 1/10 on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 h")
![Immunohistochemistry-Paraffin: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 10 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-1-10-Antibody-ST05-43-Immunohistochemistry-Paraffin-NBP2-67544-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 10 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 10 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-1-10-Antibody-ST05-43-Immunohistochemistry-Paraffin-NBP2-67544-img0003.jpg "Immunohistochemistry-Paraffin: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 10 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1/10 (washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (red) at 1/500 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-1-10-Antibody-ST05-43-Immunohistochemistry-Paraffin-NBP2-67544-img0007.jpg "Immunohistochemistry-Paraffin: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1/10 (washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (red) at 1/500 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry-Paraffin: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 1/10 ( washed with PBS. And then probed with the primary antibodies Cytokeratin 1/10 (red) at 1/500 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-1-10-Antibody-ST05-43-Immunohistochemistry-Paraffin-NBP2-67544-img0008.jpg "Immunohistochemistry-Paraffin: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 1/10 ( washed with PBS. And then probed with the primary antibodies Cytokeratin 1/10 (red) at 1/500 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry-Paraffin: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1/10 (washed with PBS. And then probed with the primary antibodies Cytokeratin 1/10 ( red) at 1/400 dilution and Vimentin (green) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-1-10-Antibody-ST05-43-Immunohistochemistry-Paraffin-NBP2-67544-img0009.jpg "Immunohistochemistry-Paraffin: Cytokeratin 1/10 Antibody (ST05-43) [NBP2-67544] - Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1/10 (washed with PBS. And then probed with the primary antibodies Cytokeratin 1/10 ( red) at 1/400 dilution and Vimentin (green) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")