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Cytochrome P450 2C8 Antibody

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Western Blot: Cytochrome P450 2C8 Antibody [NBP2-16078] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP2C8 antibody diluted at 1:1000. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: Cytochrome P450 2C8 Antibody [NBP2-16078] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP2-16078] - Antibody detects CYP2C8 protein at cytosol on hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. CYP2C8 antibody ...read more

Product Details

Summary
Reactivity Hu, RMSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Cytochrome P450 2C8 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Cytochrome P450 2C8. The exact sequence is proprietary.
Localization
Endoplasmic reticulum membrane; Microsome membrane
Predicted Species
Rhesus Macaque (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYP2C8
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Cytochrome P450 2C8 Antibody

  • CPC8
  • CYPIIC8
  • cytochrome P450 2C8
  • Cytochrome P450 form 1
  • Cytochrome P450 IIC2
  • Cytochrome P450 MP-12
  • Cytochrome P450 MP-20
  • cytochrome P450, family 2, subfamily C, polypeptide 8
  • cytochrome P450, subfamily IIC (mephenytoin 4-hydroxylase), polypeptide 8
  • EC 1.14.14.1
  • flavoprotein-linked monooxygenase
  • microsomal monooxygenase
  • MP-12/MP-20
  • P450 form 1
  • S-mephenytoin 4-hydroxylase
  • xenobiotic monooxygenase

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize many xenobiotics, including the anticonvulsive drug mephenytoin, benzo(a)pyrene, 7-ethyoxycoumarin, and the anti-cancer drug taxol. This gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CYP2C8
Uniprot