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Cystatin C Antibody [Biotin]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF1238
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF1238
Carrier Free
AF1238
Carrier Free
AF1238-SP
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Cystatin C Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cystatin C
Ala21-Ala140
Accession # P21460
Specificity
Detects mouse Cystatin C in ELISAs and Western blots. In sandwich ELISAs, approximately 30% cross-reactivity with recombinant rat Cystatin C is observed, less than 0.2% cross-reactivity with recombinant human (rh) Cystatin C is observed and less than 0.4% cross‑reactivity with recombinant mouse (rm) Cathepsin B, rmActive Cathepsin C (2336-CY), rmCathepsin C (1034-CY), rmCathepsin D, rmCathepsin E, rmCathepsin H, rmCathepsin L, rmCathepsin X/Z/P, rmCystatin B, rmCystatin E/M and rmCystatin F is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CST3
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture:Mouse Cystatin C Antibody (Catalog # AF1238)
ELISA Detection: Mouse Cystatin C Biotinylated Antibody (Catalog # BAF1238)
Standard: Recombinant Mouse Cystatin C (Catalog # 1238-PI)
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin C Antibody [Biotin]

  • ARMD11
  • bA218C14.4 (cystatin C)
  • CST3
  • cystatin 3
  • cystatin C (amyloid angiopathy and cerebral hemorrhage)
  • Cystatin C
  • cystatin-3
  • cystatin-C
  • Gamma-trace
  • MGC117328
  • Neuroendocrine basic polypeptide
  • Post-gamma-globulin

Background

Cystatin C is a member of family 2 of the cystatin superfamily (1). It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S (2, 3). All mouse tissues analyzed expressed Cystatin C, with relative levels similar to those of rat and human tissues. For all three species, brain and liver had the highest and lowest levels of Cystatin C, respectively, whereas kidney, spleen and muscle had the levels in between (4). The high degree of similarity in distribution and functional properties for mouse, rat and human Cystatin C indicates that a murine model should be relevant for studies of the human disease, hereditary Cystatin C amyloid angiopathy (4).

  1. Reed, C.H. (2000) British J. Biomed. Sci. 57:323.
  2. Janowski, R. et al. (2001) Nat. Struct. Biol. 8:316.
  3. Abrahamson, M. (1994) Methods Enzymol. 244:685.
  4. Hakansson, K. et al. (1996) Comp. Biochem. Physiol. 114B:303.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CST3
Uniprot