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Cystatin C Antibody [Unconjugated]

Name: Cystatin C Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF6154
Price: 399 USD

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Western blot shows rat plasma. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Rat Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6154) followed by HRP-conjugated Anti-Sheep IgG Secondary ...read more

Cystatin C was detected in perfusion fixed frozen sections of rat brain (cerebellum) using Sheep Anti-Rat Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6154) at 1.7 µg/mL overnight at 4 °C. ...read more

Simple Western lane view shows rat plasma, loaded at 0.2 mg/mL. A specific band was detected for Cystatin C at approximately 28 kDa (as indicated) using 10 µg/mL of Sheep Anti-Rat Cystatin C Antigen Affinity-purified ...read more

Product Details

Summary

Reactivity	RtSpecies Glossary
Applications	WB, Simple Western, IHC, IP
Clonality	Polyclonal
Host	Sheep
Conjugate	Unconjugated
Concentration	LYOPH

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Catalog# & Formulation	Size	Price
Carrier Free AF6154-SP

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Cystatin C Antibody [Unconjugated] Summary

Immunogen	Mouse myeloma cell line NS0-derived recombinant rat Cystatin C Met1-Ala140 Accession # P14841
Specificity	Detects rat Cystatin C in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse Cystatin C and approximately 15% cross-reactivity with recombinant human Cystatin C is observed.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Sheep
Gene	CST3
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Immunohistochemistry 5-15 ug/mL
Immunoprecipitation 25 ug/mL
Simple Western 10 ug/mL
Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin C Antibody [Unconjugated]

ARMD11
bA218C14.4 (cystatin C)
CST3
cystatin 3
cystatin C (amyloid angiopathy and cerebral hemorrhage)
Cystatin C
cystatin-3
cystatin-C
Gamma-trace
MGC117328
Neuroendocrine basic polypeptide
Post-gamma-globulin

Background

Cystatin C is a member of family 2 in the Cystatin superfamily (1). It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S (2, 3). It is ubiquitous in human tissues and body fluids. A point mutation in the gene coding for the 120 amino acid mature Cystatin C causes a hereditary form of amyloid angiopathy in which the protein variant is deposited in the cerebral arteries, leading to fatal cerebral hemorrhage (4). Cystatin C may have additional clinical applications. For example, it is a good marker for glomerular filtration rate (5).

Reed, C.H. (2000) British J. Biomed. Sci. 57:323.
Janowski, R. et al. (2001) Nat. Struct. Biol. 8:316.
Abrahamson, M. (1994) Methods Enzymol. 244:685.
Abrahamson, M. et al. (1992) Hum. Genet. 89:377.
Laterza, O.F. et al. (2002) Clin. Chem. 48:699.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Cystatin C
Antibody [Unconjugate

AF6154

Species: Rt

Applications: WB, Simple Western, IHC, IP

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Sheep IgG Isotype Control [Unconjugated]
5-001-A

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Bioinformatics

Gene Symbol	CST3
Uniprot	P14841()

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Cystatin C Antibody [Unconjugated]

Species Glossary

Images

Product Details

Order Details

Cystatin C Antibody [Unconjugated] Summary

Applications/Dilutions

Packaging, Storage & Formulations

Notes

Alternate Names for Cystatin C Antibody [Unconjugated]

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for Cystatin C Antibody (AF6154) (0)

Reviews for Cystatin C Antibody (AF6154) (0)

Product General Protocols

Video Protocols

FAQs for Cystatin C Antibody (AF6154) (0)

Secondary Antibodies

Isotype Controls

Additional Cystatin C Products

Blogs on Cystatin C

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics