Novus Biologicals products are now on bio-techne.com

Cystathionase Antibody

Images

 
Western Blot: Cystathionase Antibody [NBP1-52849] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.
Western Blot: Cystathionase Antibody [NBP1-52849] - CSE expression in wild type HEK293 cell (lane 1) or CSE overexpressed HEK293 (lane 2) whole cell lysate. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Cystathionase Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to CTH(cystathionase (cystathionine gamma-lyase)) The peptide sequence was selected from the N terminal of human CTH. Peptide sequence VPPISLSTTFKQGAPGQHSGFEYSRSGNPTRNCLEKAVAALDGAKYCLAF. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CTH
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation
  • Western Blot 1.0 ug/ml
Application Notes
Use in Immunoprecipitation reported in scientific literature (PMID 25900831).
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 5
using
NBP1-52849 in the following applications:

Publications
Read Publications using
NBP1-52849 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    6 publications

Reactivity Notes

Rat and Mouse reactivity reported in scientific literature (PMID: 25900831). Rat reactivity reported in scientific literature (PMID: 27531649).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for Cystathionase Antibody

  • cystathionase (cystathionine gamma-lyase)
  • cystathionine gamma-lyase
  • cysteine desulfhydrase
  • EC 4.4.1
  • EC 4.4.1.1
  • gamma-cystathionase
  • homoserine deaminase
  • homoserine dehydratase
  • MGC9471

Background

CTH is a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in its gene cause cystathioninuria.This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00000875-M01
Species: Ba, Hu, Mu, Po, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, WB
NBP1-82617
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
NBP1-82558
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
MAB5285
Species: Hu
Applications: Simple Western, WB
NBP1-81838
Species: Hu, Mu
Applications: IHC, IHC-P, WB
AF5724
Species: Hu, Mu, Rt
Applications: ICC, WB
NBP2-93574
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-87781
Species: Hu, Mu
Applications: IHC, IHC-P, WB
MAB6734
Species: Hu
Applications: IHC
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
AF1230
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
NBP1-80886
Species: Hu
Applications: Flow, IHC, IHC-P, IP, WB
NBP2-37607
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, WB
H00002729-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, WB
NB300-605
Species: Hu, Mu, Po, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB

Publications for Cystathionase Antibody (NBP1-52849)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: IF/IHC, IHC, IHC-Fr, IP, WB.


Filter By Application
IF/IHC
(1)
IHC
(1)
IHC-Fr
(1)
IP
(1)
WB
(6)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
Rat
(3)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-52849 Applications Species
Angulo J, Fern�ndez A, Sevilleja-Ortiz A et al. Upregulation of Orai Channels Contributes to Aging-Related Vascular Alterations in Rat Coronary Arteries International Journal of Molecular Sciences 2023-08-29 [PMID: 37686206] (WB) WB
Browe BM, Peng YJ, Nanduri J et al. Gasotransmitter modulation of hypoglossal motoneuron activity eLife 2023-01-19 [PMID: 36656752] (IHC-Fr, IHC) IHC-Fr, IHC
El Assar M, GarcIa-Rojo E, Sevilleja-Ortiz A Et al. Functional Role of STIM-1 and Orai1 in Human Microvascular Aging Cells 2022-11-26 [PMID: 36429103] (WB, IF/IHC, Human)

Details:
Citation using the HRP version of this antibody.
WB, IF/IHC Human
Sevilleja-Ortiz A, El Assar M, GarcIa-Rojo E Et al. Ageing-induced hypercontractility is related to functional enhancement of STIM/Orai and upregulation of Orai 3 in rat and human penile tissue Mech Ageing Dev 2021-10-26 [PMID: 34699858] (WB, Rat, Human)

Details:
Citation using the HRP version of this antibody.
WB Rat, Human
La Fuente JM, Sevilleja-Ortiz A, Garcia-Rojo E et al. Erectile dysfunction is associated with defective L-cysteine/hydrogen sulfide pathway in human corpus cavernosum and penile arteries Eur. J. Pharmacol. 2020-07-23 [PMID: 32712093] (WB, Human)

Details:
Citation using the HRP format of this antibody.
WB Human
Yuan G, Peng YJ, Khan SA et al. H2S production by reactive oxygen species in the carotid body triggers hypertension in a rodent model of sleep apnea Sci Signal 2016-08-16 [PMID: 27531649] (WB, Rat) WB Rat
Yuan G, Vasavda C, Peng YJ et al. Protein kinase G-regulated production of H2S governs oxygen sensing. Sci Signal. 2015-04-22 [PMID: 25900831] (WB, IP, Mouse, Rat) WB, IP Mouse, Rat
Madden JA, Ahlf SB, Dantuma MW, Olson KR, Roerig DL. Precursors and inhibitors of hydrogen sulfide synthesis affect acute hypoxic pulmonary vasoconstriction in the intact lung. J Appl Physiol;112(3):411-8. 2012-02-01 [PMID: 22074719]

Reviews for Cystathionase Antibody (NBP1-52849) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Other.

Reviews using NBP1-52849:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Other
(3)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
WB Other 09/08/2014
View

Summary

ApplicationWestern Blot
Sample TestedRat whole liver lysate
SpeciesOther
LotQC16215
  5
reviewed by:
Verified Customer
WB Other 05/06/2014
View

Summary

ApplicationWestern Blot
Sample TestedHEK293 whole cell lysate
SpeciesOther
LotQC16215
  5
reviewed by:
Verified Customer
WB Other 05/02/2014
View

Summary

ApplicationWestern Blot
Sample TestedHEK293 whole cell lysate
SpeciesOther
LotQC16215

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Cystathionase Antibody (NBP1-52849) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Cystathionase Products

Research Areas for Cystathionase Antibody (NBP1-52849)

Find related products by research area.

Blogs on Cystathionase

There are no specific blogs for Cystathionase, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

 
Verified Customer
09/08/2014
Application: WB
Species: Other

 
Verified Customer
05/06/2014
Application: WB
Species: Other

 
Verified Customer
05/02/2014
Application: WB
Species: Other

Bioinformatics

Gene Symbol CTH
Entrez
Uniprot