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Cystathionase Antibody (OTI1E12)

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Western Blot: Cystathionase Antibody (OTI1E12) [NBP2-03638] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cystathionase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; ...read more
Immunohistochemistry-Paraffin: Cystathionase Antibody (OTI1E12) [NBP2-03638] - Staining of paraffin-embedded Human pancreas tissue using anti-Cystathionase mouse monoclonal antibody.
Western Blot: Cystathionase Antibody (OTI1E12) [NBP2-03638] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cystathionase (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunohistochemistry-Paraffin: Cystathionase Antibody (OTI1E12) [NBP2-03638] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Cystathionase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cystathionase Antibody (OTI1E12) [NBP2-03638] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Cystathionase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cystathionase Antibody (OTI1E12) [NBP2-03638] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Cystathionase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cystathionase Antibody (OTI1E12) [NBP2-03638] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Cystathionase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cystathionase Antibody (OTI1E12) [NBP2-03638] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Cystathionase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cystathionase Antibody (OTI1E12) [NBP2-03638] - Staining of paraffin-embedded Human Kidney tissue using anti-Cystathionase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cystathionase Antibody (OTI1E12) [NBP2-03638] - Staining of paraffin-embedded Human liver tissue using anti-Cystathionase mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Ca, Pm, PmSpecies Glossary
Applications WB, IHC
Clone
OTI1E12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Cystathionase Antibody (OTI1E12) Summary

Immunogen
Full length human recombinant protein of human CTH (NP_001893) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CTH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
44.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Cystathionase Antibody (OTI1E12)

  • cystathionase (cystathionine gamma-lyase)
  • cystathionine gamma-lyase
  • cysteine desulfhydrase
  • EC 4.4.1
  • EC 4.4.1.1
  • gamma-cystathionase
  • homoserine deaminase
  • homoserine dehydratase
  • MGC9471

Background

This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CTH
Entrez